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Anti-SIAH1/2 antibody [8G7H12] (ab77167)

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Overview

Product name

Anti-SIAH1/2 antibody [8G7H12]

Description

Mouse monoclonal [8G7H12] to SIAH1/2

Specificity

ab77167 is specific for human SIAH1 and SIAH2 and Drosophila SINA in proliferating cells and stem cells. This antibody does not detect Drosophila SINAH or normal, nondividing cells.

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Rat, Human, Pig, Fruit fly (Drosophila melanogaster), Zebrafish

Does not react with

Mouse

Immunogen

Synthetic peptide made to an C terminal portion of the drosophila SINA protein (within residues 280-331) (NP_005058.2).

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

8G7H12

Isotype

IgG1

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Drug resistance >> MRP-related proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/5Detects a band of...Read more →

    WB: 1/5Detects a band of approximately 37 kDa (predicted molecular weight: 31-35 kDa).

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

Target

Relevance

These genes encode a proteins that are members of the seven in absentia homolog (SIAH) family. The proteins are E3 ligases and are involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of these ubiquitin ligases have been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis.

Cellular localization

Cytoplasmic and Nuclear

Alternative names

  • E3 ubiquitin protein ligase SIAH2 antibody
  • hSIAH1 antibody
  • hSiah2 antibody
  • HUMSIAH antibody
  • Seven in absentia homolog 1 (Drosophila) antibody
  • Seven in absentia homolog 1 antibody
  • Seven in Absentia Homolog 2 antibody
  • SIAH 1 antibody
  • Siah 1a antibody
  • Siah 2 antibody
  • Ubiquitin ligase SIAH1 antibody
  • Ubiquitin Ligase Siah2 antibody
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Database links

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References for Anti-SIAH1/2 antibody [8G7H12] (ab77167)

This product has been referenced in:

  • Schmidt RLet al. Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue. Cancer Res 67:11798-810 (2007).Read more (PubMed: 18089810) »

See 1 publication for this product

Publishing research using ab77167? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"