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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-SIAH1 antibody
See all SIAH1 products (6) ...
Description
Rabbit polyclonal to SIAH1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
A region within synthetic peptide: MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP, corresponding to N terminal amino acids 1-50 of Human SIAH1
MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - SIAH1 antibody (ab49146)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
(ELISA titre using peptide based assay: 1:312500)
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Cellular localization
Predominantly cytoplasmic, but also nuclear
Alternative names
- hSIAH1 antibody
- HUMSIAH antibody
- Seven in absentia homolog 1 (Drosophila) antibody
see all
Database links
- Entrez Gene: 6477 Human
- Entrez Gene: 20437 Mouse
- Entrez Gene: 140941 Rat
- Entrez Gene: 80955 Zebrafish
- Omim: 602212 Human
- SwissProt: Q8IUQ4 Human
- SwissProt: P61092 Mouse
- SwissProt: Q920M9 Rat
see all
Anti-SIAH1 antibody images:
Western blot - SIAH1 antibody (ab49146)
All lanes : Anti-SIAH1 antibody (ab49146) at 0.5 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 1 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 35 kDa
References for Anti-SIAH1 antibody (ab49146)
ab49146 has not yet been referenced specifically in any publications.
Publishing research using ab49146? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"