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Anti-SIAH1 antibody (ab49177) at 1.25 µg/ml + Human placenta lysate at 10 µg with 5% skimmed milk in PBS
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
developed using the ECL technique
Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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