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Anti-SIAH1 antibody (ab49177)

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Overview

Product name

Anti-SIAH1 antibody
See all SIAH1 products (6) ...

Description

Rabbit polyclonal to SIAH1

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Dog, Fruit fly (Drosophila melanogaster), Zebrafish

Immunogen

A region within synthetic peptide: QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL, corresponding to C terminal amino acids 217-266 of Human SIAH1

QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Placenta lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway

  • Western blot - SIAH1 antibody (ab49177)Western blot - SIAH1 antibody (ab49177) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:312500
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Cellular localization

Predominantly cytoplasmic, but also nuclear

Database links

see all

Anti-SIAH1 antibody images:

  Western blot - SIAH1 antibody (ab49177)

Western blot - SIAH1 antibody (ab49177)

Anti-SIAH1 antibody (ab49177) at 1.25 µg/ml + Human placenta lysate at 10 µg with 5% skimmed milk in PBS

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
developed using the ECL technique

Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-SIAH1 antibody (ab49177)

ab49177 has not yet been referenced specifically in any publications.

Publishing research using ab49177? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"