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All lanes : Anti-SIAH1 antibody (ab69638) at 1/500 dilution
Lane 1 : Extracts from A549 cell lysate
Lane 2 : Extracts from HeLa cell lysate
Lane 3 : Extracts from A549 cell lysate with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
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