You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-SIAH1 antibody (ab69638)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-SIAH1 antibody
See all SIAH1 products (6) ...

Description

Rabbit polyclonal to SIAH1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthesized peptide derived from internal of human SIAH1.

Positive control

Extracts form A549 and HeLa cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway

  • Western blot - SIAH1 antibody (ab69638)Western blot - SIAH1 antibody (ab69638) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Relevance

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Cellular localization

Predominantly cytoplasmic, but also nuclear

Alternative names

  • hSIAH1 antibody
  • HUMSIAH antibody
  • Seven in absentia homolog 1 (Drosophila) antibody
  • Seven in absentia homolog 1 antibody
  • Siah 1 antibody
  • Siah 1a antibody
  • Ubiquitin ligase SIAH1 antibody
see all

Database links

Anti-SIAH1 antibody images:

  Western blot - SIAH1 antibody (ab69638)

Western blot - SIAH1 antibody (ab69638)

All lanes : Anti-SIAH1 antibody (ab69638) at 1/500 dilution

Lane 1 : Extracts from A549 cell lysate
Lane 2 : Extracts from HeLa cell lysate
Lane 3 : Extracts from A549 cell lysate with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-SIAH1 antibody (ab69638)

ab69638 has not yet been referenced specifically in any publications.

Publishing research using ab69638? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"