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Western blot - SIAH1 antibody (ab69638)
(enlarge)
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Product Name
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SIAH1 antibody
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See all SIAH1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SIAH1
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Immunogen
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Synthesized peptide derived from internal of human SIAH1.
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Reacts with
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts form A549 and HeLa cells.
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Cellular localization
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Predominantly cytoplasmic, but also nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SIAH1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SIAH1 antibody images, references, products related to ab69638 and other tools.
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SIAH1 antibody images:
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 Western blot - SIAH1 antibody (ab69638)
All lanes : SIAH1 antibody (ab69638) at 1/500 dilution
Lane 1 : Extracts from A549 cell lysate
Lane 2 : Extracts from HeLa cell lysate
Lane 3 : Extracts from A549 cell lysate with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to SIAH1
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SIAH1 antibody - more information
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Customer reviews (feedback) regarding SIAH1 antibody |
Customer FAQs regarding SIAH1 antibody |
Protocols for SIAH1 antibody |
Price and availability of products related to SIAH1 antibody |
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SIAH1 antibody - related products:
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SIAH1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SIAH1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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