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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-SIGIRR antibody
See all SIGIRR products (7) ...
Description
Rabbit polyclonal to SIGIRR
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) Human.
IRGRVPEGRA LDSEVDPDPE GDLGV
Positive control
Human or mouse kidney cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Immunology >> Immunoglobulins >> Receptors
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Western blot - TIR8 antibody (ab22053)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Flow Cyt
Application notes
Flow Cyt: Use 1µg for 106 cells.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 45 kDa in human kidney cell lysate, and a band of approximately 50 kDa in mouse kidney cell lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
Tissue specificity
Mainly expressed in epithelial tissues such as kidney, lung and gut.
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 TIR domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ6IA17
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Single immunoglobulin domain containing IL1R related protein antibody
- TIR8 antibody
- Toll/interleukin 1 receptor 8 antibody
see all
Database links
- Entrez Gene: 59307 Human
- Entrez Gene: 24058 Mouse
- Omim: 605478 Human
- SwissProt: Q6IA17 Human
- SwissProt: Q9JLZ8 Mouse
- Unigene: 501624 Human
- Unigene: 38017 Mouse
Anti-SIGIRR antibody images:
Western blot - TIR8 antibody (ab22053)
All lanes : Anti-SIGIRR antibody (ab22053) at 0.5 µg/ml
Lane 1 : human kidney cell lysate
Lane 2 : mouse kidney cell lysate
Lysates/proteins at 15 µg per lane.
Observed band size : 45,50 kDa (why is the actual band size different from the predicted?)
References for Anti-SIGIRR antibody (ab22053)
This product has been referenced in:
- Palaniappan Ret al. CCL5 Modulates Pneumococcal Immunity and Carriage. J Immunol 176:2346-56 (2006).Read more (PubMed: 16455992) »
See 1 publication for this product
Publishing research using ab22053? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"