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Western blot - SIGIRR antibody (ab25875)
(enlarge)
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Immunohistochemistry - SIGIRR antibody (ab25875)
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Product Name
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SIGIRR antibody
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See all SIGIRR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SIGIRR
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Immunogen
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Synthetic peptide corresponding to 15 amino acids at the C terminus of Mouse SIGIRR.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab25875 recognises SIGIRR.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A549 cell lysate
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Cellular localization
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Membrane; Single pass type III membrane protein
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Immunology >> Innate Immunity >> Cytokines >> Interleukins
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Relevance
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SIGIRR is a novel member of the Interleukin 1 receptor (IL1R) superfamily. Although it shows the typical conserved motifs that characterize the IL1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR has only one Ig domain in its extracellular portion whereas the IL1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of Drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL1RI. A chimera, in which the SIGIRR intracellular domain was fused to the IL1R extracellular domain, did not activate NFkappaB unlike similar fusion proteins of other IL1R related molecules. Phylogenetic data suggest that the plasma membrane protein SIGIRR is a close functional relative of MyD88 in mammals.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SIGIRR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SIGIRR antibody images, references, products related to ab25875 and other tools.
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SIGIRR antibody images:
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 Western blot - SIGIRR antibody (ab25875)
Predicted band size : 47 kDa
Western blot analysis of SIGIRR in A549 cell lysate with anti-SIGIRR (CT) at (A) 0.5, (B) 1, and (C) 2 ug/ml.
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Immunohistochemistry - SIGIRR antibody (ab25875)
ab25875 at 10µg/ml in A549 staining SIGIRR in A549 cells by ICC/IF
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Search PubMed (MEDLINE) for references to SIGIRR
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SIGIRR antibody - more information
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Customer reviews (feedback) regarding SIGIRR antibody |
Customer FAQs regarding SIGIRR antibody |
Protocols for SIGIRR antibody |
Price and availability of products related to SIGIRR antibody |
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SIGIRR antibody - related products:
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SIGIRR antibody - other tools:
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Contact Abcam with a Technical Enquiry about SIGIRR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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