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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-SIGIRR antibody
See all SIGIRR products (7) ...
Description
Rabbit polyclonal to SIGIRR
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SIGIRR.
(Peptide available as ab75025.)
Positive control
This antibody gave a positive signal in the following lysates: Kidney (Mouse) Tissue, HepG2 Whole Cell, SK N SH Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Immunology >> Immunoglobulins >> Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
Target
Function
Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
Tissue specificity
Mainly expressed in epithelial tissues such as kidney, lung and gut.
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 TIR domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ6IA17
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Single immunoglobulin domain containing IL1R related protein antibody
- TIR8 antibody
- Toll/interleukin 1 receptor 8 antibody
see all
Database links
- Entrez Gene: 59307 Human
- Entrez Gene: 24058 Mouse
- Omim: 605478 Human
- SwissProt: Q6IA17 Human
- SwissProt: Q9JLZ8 Mouse
- Unigene: 501624 Human
- Unigene: 38017 Mouse
Anti-SIGIRR antibody images:
Western blot - SIGIRR antibody (ab49573)
All lanes : Anti-SIGIRR antibody (ab49573) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 6 minutes
SIGIRR contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 27 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SIGIRR.
Immunocytochemistry/ Immunofluorescence - SIGIRR antibody (ab49573)
ICC/IF image of ab49573 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, hepG2 and MCF7 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SIGIRR antibody (ab49573)
IHC image of SIGIRR staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49573, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-SIGIRR antibody (ab49573)
ab49573 has not yet been referenced specifically in any publications.
Publishing research using ab49573? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"