You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameSIGLEC5 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    6.86 pg/ml - 5000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.3 91% - 108%
    Serum 106.3 93% - 114%
    Plasma 102.4 91% - 111%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100641 SIGLEC5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIGLEC5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human SIGLEC5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC5present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SIGLEC5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    400X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Siglec-5 : biotinylated anti-human Siglec-5 2 vials
    Siglec 5 Microplate (12 strips x 8 wells) coated with anti-human Siglec-5 1 unit
    Siglec 5 Standard Human (recombinant) 2 vials
    Stop Solution Item I 1 x 25ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevancePutative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
    • Alternative names
        CD170CD170 antigen CD33 antigen like 2
        CD33L2mSiglec FOB binding protein 2OB BP2 OBBP2Obesity binding protein 2OTTMUSP00000041735OTTMUSP00000041736Sialic acid binding Ig like lectin 5Sialic acid binding immunoglobulin like lectin 5SIGLEC 5Siglec FSiglec F variant Siglecf
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100641 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      SIGLEC5 Human ELISA Kit images

      • ELISA - SIGLEC5 Human ELISA Kit (ab100641)
        ELISA - SIGLEC5 Human ELISA Kit (ab100641)

      Protocols

      References for SIGLEC5 Human ELISA Kit (ab100641)

      ab100641 has not yet been referenced specifically in any publications.

      Publishing research using ab100641 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100641 Submit a Question for ab100641

      There are currently no Abreviews or Questions for ab100641.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"