SIM1 antibody (ab21932)

Product Name 

SIM1 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to SIM1

Immunogen 

Fusion protein: SKPTFSYTSS STPTISDNRK GAKSRLSSSK SKSRTSPYPQ YSGFHTERSE SDHDSQWGGS PLTDTASPQL LDPERPGSQH ELSCAYRQFP DRSSLCYGFA corresponding to amino acids 349/448 of Mouse SIM1.
BLAST 'SKPTFSYTSS STPTISDNRK GAKSRLSSSK SKSRTSPYPQ YSGFHTERSE SDHDSQWGGS PLTDTASPQL LDPERPGSQH ELSCAYRQFP DRSSLCYGFA ' with or

Reacts with 
(species key)

Ms

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 85 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Cellular localization 

Nuclear

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neural Signal Transduction

Relevance 

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.

SIM1 is a transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Constituents: 50% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Produced from outbred CD1 mice

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