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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-SIM1 antibody
See all SIM1 products (2) ...
Description
Mouse polyclonal to SIM1
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
Fusion protein: SKPTFSYTSSSTPTISDNRKGAKSRLSSSKSKSRTSPYPQ YSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQH ELSCAYRQFPDRSSLCYGFA, corresponding to amino acids 349/448 of Mouse SIM1.
SKPTFSYTSS STPTISDNRK GAKSRLSSSK SKSRTSPYPQ YSGFHTERSE SDHDSQWGGS PLTDTASPQL LDPERPGSQH ELSCAYRQFP DRSSLCYGFA
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - SIM1 antibody (ab21932)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 85 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome. SIM1 is a transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
Cellular localization
Nuclear
Alternative names
- BHLHE14 antibody
- Class E basic helix-loop-helix protein 14 antibody
- Single minded homolog 1 antibody
Database links
- Entrez Gene: 20464 Mouse
- SwissProt: Q61045 Mouse
- Unigene: 4774 Mouse
Anti-SIM1 antibody images:
Western blot - SIM1 antibody (ab21932)
All lanes : Anti-SIM1 antibody (ab21932) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 85 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-SIM1 antibody (ab21932)
ab21932 has not yet been referenced specifically in any publications.
Publishing research using ab21932? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"