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Overview

  • Product nameSIN1 protein (Tagged-His Tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-330 of Human SIN1 (Isoform 4) with N terminal His tag, 55kDa (37.3kDa predicted from sequence). UniProt ID = Q9BPZ7.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 78 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCL HIAEDDGEVDTDFPPLDSN EPIHKFGFSTLALVEKYSS PGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVK RRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWE FCLVRENSSRADGVFEEDSQI DIATVQDMLSSHHYKSF KVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIK QK PISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYK HLYFESDAATVNEIVLKVNYILE SRASTARADYFAQKQ RKLNRRTSFSFQKEKKSGQQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91839 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        MAPKAP 1MAPKAP1MEKK2 interacting protein 1
        MGC2745MIP 1MIP1Mitogen activated protein kinase associated protein 1Mitogen-activated protein kinase 2-associated protein 1mSIN1OTTHUMP00000064207Ras inhibitor MGC2745SAPK interacting protein 1SAPK-interacting protein 1SIN 1SIN1_HUMANSIN1bSIN1gStress activated map kinase interacting protein 1Stress activated protein kinase interacting 1Stress-activated map kinase-interacting protein 1Target of rapamycin complex 2 subunit MAPKAP1TORC2 subunit MAPKAP1
      see all
  • FunctionSubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
  • Tissue specificityUbiquitously expressed, with highest levels in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the SIN1 family.
  • Cellular localizationCell membrane. Cytoplasmic vesicle. Nucleus.

    • Target information above from: UniProt accession Q9BPZ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SIN1 protein (Tagged-His Tag) images

    • SDS-PAGE - SIN1 protein (Tagged-His Tag) (ab91839)
      SDS-PAGE - SIN1 protein (Tagged-His Tag) (ab91839)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SIN1 protein (Tagged-His Tag) (ab91839)

    ab91839 has not yet been referenced specifically in any publications.

    Publishing research using ab91839 ? Please let us know so that we can cite the reference in this datasheet

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