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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab10162? Please let us know so that we can cite the reference in this datasheet
ab10162 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SIPA1 antibody (ab10162)
Predicted band size : 130 kDa
20µg of B cell lysate from different patients with Chronic Lymphocytic Leukaemia (a B cell malignancy) were separated by SDS-PAGE and blotted with ab10162 for 1 hour (1/500 dilution). The levels of SIPA1 in patients with CLL was compared to that in normal B cells (end lane).
Spa-1 is the Rap1-GAP of lympocytes, and therefore all the samples should all possess Spa-1. Equal protein was loaded in each lane. A band of 130kDa was detected in all samples (arrow). Non-specific bands of ~ 60kDa were also detected.
This image was submitted as part of a review by Kathy Till
SIPA1 antibody for WB in Human (10162)
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