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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SIPA1 antibody (ab10162)
Predicted band size : 130 kDa
20µg of B cell lysate from different patients with Chronic Lymphocytic Leukaemia (a B cell malignancy) were separated by SDS-PAGE and blotted with ab10162 for 1 hour (1/500 dilution). The levels of SIPA1 in patients with CLL was compared to that in normal B cells (end lane).
Spa-1 is the Rap1-GAP of lympocytes, and therefore all the samples should all possess Spa-1. Equal protein was loaded in each lane. A band of 130kDa was detected in all samples (arrow). Non-specific bands of ~ 60kDa were also detected.
This image was submitted as part of a review by Kathy Till
SIPA1 antibody for WB in Human (10162)
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