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Western blot - SIPA1 antibody (ab10162)
(enlarge)
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Product Name
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SIPA1 antibody
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See all SIPA1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SIPA1
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Immunogen
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Synthetic peptide: ASKQLGSPTADLA, corresponding to C terminal amino acids 1030-1042 of Human SIPA1.
BLAST 'C-ASKQLGSPTADLA' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at an assay dependent dilution. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Human lymph node and Jurkat.
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Cellular localization
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Cytoplasmic and Nuclear (Perinuclear)
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SIPA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SIPA1 antibody images, references, products related to ab10162 and other tools.
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SIPA1 antibody images:
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 Western blot - SIPA1 antibody (ab10162)
Predicted band size : 130 kDa
20µg of B cell lysate from different patients with Chronic Lymphocytic Leukaemia (a B cell malignancy) were separated by SDS-PAGE and blotted with ab10162 for 1 hour (1/500 dilution). The levels of SIPA1 in patients with CLL was compared to that in normal B cells (end lane). Spa-1 is the Rap1-GAP of lympocytes, and therefore all the samples should all possess Spa-1. Equal protein was loaded in each lane. A band of 130kDa was detected in all samples (arrow). Non-specific bands of ~ 60kDa were also detected. This image was submitted as part of a review by Kathy Till
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Search PubMed (MEDLINE) for references to SIPA1
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SIPA1 antibody - more information
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Customer reviews (feedback) regarding SIPA1 antibody |
Customer FAQs regarding SIPA1 antibody |
Protocols for SIPA1 antibody |
Price and availability of products related to SIPA1 antibody |
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SIPA1 antibody - related products:
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SIPA1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SIPA1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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