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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-SIPA1 antibody
See all SIPA1 products (3) ...
Description
Goat polyclonal to SIPA1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ASKQLGSPTADLA, corresponding to C terminal amino acids 1030-1042 of Human SIPA1.
C-ASKQLGSP TADLA
Positive control
Human lymph node and Jurkat.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - SIPA1 antibody (ab10162)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
Target
Function
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Tissue specificity
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Sequence similarities
Contains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Cellular localization
Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
Target information above from: UniProt accessionQ96FS4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GTPase activating protein Spa 1 antibody
- GTPase-activating protein Spa-1 antibody
- MGC102688 antibody
see all
Database links
- Entrez Gene: 6494 Human
- Omim: 602180 Human
- SwissProt: Q96FS4 Human
- Unigene: 530477 Human
Anti-SIPA1 antibody images:
Western blot - SIPA1 antibody (ab10162)
Predicted band size : 130 kDa
20µg of B cell lysate from different patients with Chronic Lymphocytic Leukaemia (a B cell malignancy) were separated by SDS-PAGE and blotted with ab10162 for 1 hour (1/500 dilution). The levels of SIPA1 in patients with CLL was compared to that in normal B cells (end lane).
Spa-1 is the Rap1-GAP of lympocytes, and therefore all the samples should all possess Spa-1. Equal protein was loaded in each lane. A band of 130kDa was detected in all samples (arrow). Non-specific bands of ~ 60kDa were also detected.
This image was submitted as part of a review by Kathy Till
References for Anti-SIPA1 antibody (ab10162)
ab10162 has not yet been referenced specifically in any publications.
Publishing research using ab10162? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"