![Western blot - SIRT1 antibody [E104] (ab32441) image](#ab32441wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441) image](#ab32441ihc1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441) image](#ab32441ihc2.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody [E104]
See all SIRT1 products (14) ...
Description
Rabbit monoclonal [E104] to SIRT1
Specificity
The antibody does not cross-react with other sirtuin family members.
Tested applications
IHC-Fr, WB, ICC, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in C-terminus of human Sir2/SIRT1.
Positive control
WB: Jurkat cell lysate IHC: human colon carcinoma and human lung squamous carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E104
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21098725)
WB: 1/5000Detects a band of approximately 110 kDa (predicted molecular weight: 82 kDa).(Detects a band of approximately 110 kDa (110-121 kDa) which is likely to be due to post translational glycosylation. SIRT1 is known to bind to several other proteins, and the 121kDa band could also be due to the presence of one of these complexes (ensure samples are adequately reduced and denatured).)
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
IP
IP: 1/50IP: 1/50
IHC-P: 1/100 - 1/200.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- BA57G10.4 antibody
- hSIR2 antibody
see all
Database links
- Entrez Gene: 23411 Human
- Omim: 604479 Human
- SwissProt: Q96EB6 Human
- Unigene: 369779 Human
Anti-SIRT1 antibody [E104] images:
Western blot - SIRT1 antibody [E104] (ab32441)
![Western blot - SIRT1 antibody [E104] (ab32441)](/ps/datasheet/Images/32/ab32441/ab32441wb.gif)
Anti-SIRT1 antibody [E104] (ab32441) at 1/5000 dilution + Jurkat cell lysate
Predicted band size : 82 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441)
![Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441)](/ps/datasheet/Images/32/ab32441/ab32441ihc1.jpg)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab32441 at 1/100 dilution.
Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441)
![Immunohistochemistry (Paraffin-embedded sections) - SIRT1 antibody [E104] (ab32441)](/ps/datasheet/Images/32/ab32441/ab32441ihc2.jpg)
Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32441 at 1/100 dilution.
References for Anti-SIRT1 antibody [E104] (ab32441)
This product has been referenced in:
- Sestito Ret al. STAT3-dependent effects of IL-22 in human keratinocytes are counterregulated by sirtuin 1 through a direct inhibition of STAT3 acetylation. FASEB J 25:916-27 (2011). IHC-Fr; Human.Read more (PubMed: 21098725) »
- Orecchia Aet al. Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells. PLoS One 6:e24307 (2011). IHC-P; Human.Read more (PubMed: 21931678) »
See all 8 publications for this product
Publishing research using ab32441? Please let us know so that we can cite the reference in this datasheet
![SIRT1 antibody [E104] for WB in Human (32441)](/ps/Reviews/Images/ab9817_50060.jpg)
![Anti-SIRT1 antibody [E104] for Western blot in Human (32441)](/ps/datasheet/images/32/ab32441/SIRT1-Primary-antibodies-ab32441-11.png)
![SIRT1 antibody [E104] for ICC/IF in Human (32441)](/ps/Reviews/Images/ab6757_25806.jpg)
![SIRT1 antibody [E104] for IHC-P in Human (32441)](/ps/Reviews/Images/ab7168_29045.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"