SIRT1 antibody [E104] (ab32441)

Product Name 

SIRT1 antibody [E104]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E104] to SIRT1

Immunogen 

A synthetic peptide corresponding to residues in C-terminus of human Sir2/SIRT1.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

The antibody does not cross-react with other sirtuin family members.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/5000. Detects a band of approximately 110 kDa (110-121 kDa) which is likely to be due to post translational glycosylation. SIRT1 is known to bind to several other proteins, and the 121kDa band could also be due to the presence of one of these complexes (ensure samples are adequately reduced and denatured). (predicted molecular weight: 82 kDa).

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Jurkat cell lysate
IHC: human colon carcinoma and human lung squamous carcinoma tissue.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction

Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway

Relevance 

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.
SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E104

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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SIRT1 antibody [E104] images:

References for SIRT1 antibody [E104] (ab32441)

  This product has been used in: (two most recent references)

Jung-Hynes B et al. Role of sirtuin histone deacetylase SIRT1 in prostate cancer. A target for prostate cancer management via its inhibition? J Biol Chem 284:3823-32 (2009). IP; Human. PubMed: 19075016

Menghini R et al. MicroRNA 217 modulates endothelial cell senescence via silent information regulator 1. Circulation 120:1524-32 (2009). PubMed: 19786632

See all 3 publication references for this product.


If you publish research using ab32441 please let us know so that we can cite the reference on this datasheet.

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