Anti-SIRT1 antibody [E54] (ab32424)

Thanks for your reply. There are several serines between amino acid 25-55 of human sirt1. To my knowledge, at least it has been shown that serine 47 can be phosphorylated. Do you have an idea whether this antibody can recognize both unphosphorylated and phosphorylated forms of sirt1?

ANSWER:

Thank you for your reply.

This antibody will only recognize the non-phosphorylated form of sirt1. If you are looking for an antibody against the phosphorylated form of the protein, then there are links to 2 below that will only recognize the phosphorylated form:

http://www.abcam.com/SIRT1-phospho-S27-antibody-EP2850Y-ab76029.html.
http://www.abcam.com/SIRT1-phospho-S47-antibody-EPR2849Y-ab76039.html



If there is anything else I can help you with, please let me know.

Have a great weekend.

I got a question regarding your anti-SIRT1 antibody (E54), and the catalog number is ab32424. It says that it is against residues near N-terminus and before Ala-rich domain. Would you be able to tell me the exact sequence that you have used to generate this antibody?

ANSWER:

Thank you for contacting Abcam.



The peptide maps between amino acids ******** of human Sir2/SirT1 (UniProt: Q96EB6).



Please let me know if there is anything else I can help you with.

I got a question regarding your anti-SIRT1 antibody (E54), and the catalog number is ab32424. It says that it is against residues near N-terminus and before Ala-rich domain. Would you be able to tell me the exact sequence that you have used to generate this antibody?

ANSWER:

Thank you for contacting Abcam.

Unfortunately the exact immunogen sequence for this antibody is considered to be proprietary and so I am unable to provide that sequence to you.

If there is anything else I can help you with, please let me know.

Hi, What is the concentration of the SIRT1 antibody (Cat. ab32424, lot: GR56401-2)? I was trying to use this antibody for intracellular staining, and got high background. Since you have intracellular staining data using this antibody on your website, I was just wondering if you can give me a detailed protocol. Thanks a lot and have a nice day!

ANSWER:

Thank you for contacting Abcam. The concentration for ab32424, lot#GR56401-2 is 0****** mg/mL. I am sorry about the problems that you are having with this antibody in staining, below is the only protocol we have for using ab32424 in IF: ICC/IF image of ab32424 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32424, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. If the above information does not prove helpful, please could you send me a copy of the protocol that you are using and I would be happy to see if I can make any suggestions. Please let me know if there is anything else I can help you with.

Phone call: customer kindly asked for anti SIRT1, SIRT2 and SIRT6 antibodies that can used in immunohistochemistry.

ANSWER:

Thank you for contacting us.

SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse and rat. Not yet tested in Immunohistochemistry. 

ab105391, cross reacts with human, mouse and rat. Not yet tested in Immunohistochemistry. These antibodies are not yet tested in Immunohistochemistry. However this does not mean that these are not suitable for IHC it only mean we do not have tested data to confirm their use. If you are interested in testing one of these in IHC then let me know I will send you the Abreview discount code;  which states we will offer a next free antibody in exchange of an antibody review.   

SIRT1 ab32441 and ab32424 These antibodies are tested in IHC so are fully guaranteed to work in IHC. The mouse and rat species is predicted which is based on sequence homology of immunogen between these species. If you are interested in using these antibodies in mouse and rat then let me know I will provide you the Abreview discount code which will give you your next purchase of an antibody free of charge. ab75435 and ab110304 does cross react with human, mouse and rat however not tested in IHC therefore these are eligible for Abreview discount programme.  

SIRT2 ab115681 is tested in human and mouse and in IHC-P. ab67299 and ab75436 are tested for cross reactivity in human, mouse and rat however not tested in IHC. These products are also eligible for Abreview discount programme.

I also have attached a spreadsheet of all anti SIRT6, SIRT2 and SIRT1 antibodies with Immunogen sequence. Please have a look and decide which one best suits you.

For information about publications and images please check the datasheets of individual antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.