![Western blot - SIRT1 antibody [E54] (ab32424) image](#ab32424wb.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SIRT1 antibody [E54] (ab32424) image](#ab32424_1.JPG)
![Flow Cytometry - SIRT1 antibody [E54] (ab32424) image](#ab32424_2.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody [E54]
See all SIRT1 products (14) ...
Description
Rabbit monoclonal [E54] to SIRT1
Specificity
The antibody does not cross-react with other sirtuin family members.
Tested applications
ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near N-terminus and before Ala-rich domain of human Sir2.
Positive control
Hela cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E54
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100
WB: 1/1000Predicted molecular weight: 82 kDa.
-
IHC-P: 1/50(For staining human squamous carcimoma tissue, ab32441 has given better staining results.)
Flow Cyt: 1/30
IP
IP: 1/40IP: 1/40
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- BA57G10.4 antibody
- hSIR2 antibody
see all
Database links
- Entrez Gene: 23411 Human
- Omim: 604479 Human
- SwissProt: Q96EB6 Human
- Unigene: 369779 Human
Anti-SIRT1 antibody [E54] images:
Western blot - SIRT1 antibody [E54] (ab32424)
![Western blot - SIRT1 antibody [E54] (ab32424)](/ps/datasheet/Images/32/ab32424/ab32424wb.gif)
Anti-SIRT1 antibody [E54] (ab32424) at 1/1000 dilution + Hela cell lysate
Predicted band size : 82 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SIRT1 antibody [E54] (ab32424)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SIRT1 antibody [E54] (ab32424)](/ps/datasheet/Images/32/ab32424/ab32424_1.JPG)
Immunohistochemical staining of paraffin-embedded human lymphoma using ab32424 at 1/50 dilution.
Flow Cytometry - SIRT1 antibody [E54] (ab32424)
![Flow Cytometry - SIRT1 antibody [E54] (ab32424)](/ps/datasheet/Images/32/ab32424/ab32424_2.jpg)
Flow cytometry analysis of permeabilized Hela cells using anti-SIRT1 (red)(ab32424) or a rabbit IgG (negative)(green).
Immunocytochemistry/ Immunofluorescence - SIRT1 antibody [E54] (ab32424)
![Immunocytochemistry/ Immunofluorescence - SIRT1 antibody [E54] (ab32424)](/ps/datasheet/images/32/ab32424/SIRT1-Primary-antibodies-ab32424-1.jpg)
ICC/IF image of ab32424 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32424, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SIRT1 antibody [E54] (ab32424)
This product has been referenced in:
- Zhao Yet al. Corepressive action of CBP on androgen receptor transactivation in pericentric heterochromatin in a Drosophila experimental model system. Mol Cell Biol 29:1017-34 (2009). WB; Human.Read more (PubMed: 19075001) »
See 1 publication for this product
Publishing research using ab32424? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"