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ab12193 has been referenced in 7 publications.
Publishing research using ab12193? Please let us know so that we can cite the reference in this datasheet
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Anti-SIRT1 antibody (ab12193) at 1/500 dilution (for 1 hour at 21°C) + Whole tissue lysate of mouse jejunum and ileum at 10 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 80 kDa
Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Blocking Step: 5 µg/mL milk for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
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