SIRT1 antibody (ab12193)

Product Name 

SIRT1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SIRT1

Immunogen 

Synthetic peptide: ATRQELTDV NYPSDKS (with C-terminal added lysine), corresponding to C terminal amino acids 722-737 of Mouse SIRT1
BLAST 'ATRQELTDV NYPSDKS' with or

Reacts with 
(species key)

Ms

Specificity 

Anti-SIR2 specifically recognizes mouse SIR2 by immunoblotting and immunofluorescence.

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IF: 1/50 (determined by indirect immunofluorescent staining of methanol fixed cultured mouse 3T3-NIH cells).
WB: 1/2000 (nuclear extract of mouse 3T3-NIH cells). Observed molecular weight: 110 kDa. (Predicted molecular weight: 80kDa.) In some preparations additional lower bands may be detected.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse 3T3-NIH cells.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Chromatin and Nuclear Signaling >> Chromosome Structure >> Telomeres

Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway

Relevance 

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.
SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody is affinity-purified using the immunogen peptide immobilized on agarose.

Form 

Liquid

Concentration 

Batch dependent within range: 1.10 - 1.10 mg/ml

Concentration of lot no.     is  

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.

References for SIRT1 antibody (ab12193)

  This product has been used in:

Pfluger PT et al. Sirt1 protects against high-fat diet-induced metabolic damage. Proc Natl Acad Sci U S A 105:9793-8 (2008). WB; Mouse. PubMed: 18599449

If you publish research using ab12193 please let us know so that we can cite the reference on this datasheet.

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