SIRT1 antibody (ab28170)

Product Name 

SIRT1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SIRT1

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SIRT1.

(Peptide available as ab28308.)

Reacts with 
(species key)

Ms

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction

Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway

Relevance 

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.
SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.600 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for SIRT1 antibody images, references, products related to ab28170 and other tools.

SIRT1 antibody images:

References for SIRT1 antibody (ab28170)

  This product has been used in: (two most recent references)

Csiszar A et al. Resveratrol induces mitochondrial biogenesis in endothelial cells. Am J Physiol Heart Circ Physiol 297:H13-20 (2009). PubMed: 19429820

Gupta MP et al. HDAC4 and PCAF Bind to Cardiac Sarcomeres and Play a Role in Regulating Myofilament Contractile Activity. J Biol Chem 283:10135-46 (2008). WB; Mouse. PubMed: 18250163
If you publish research using ab28170 please let us know so that we can cite the reference on this datasheet.

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