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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody
See all SIRT1 products (14) ...
Description
Rabbit polyclonal to SIRT1
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SIRT1.
(Peptide available as ab28308.)
Positive control
F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
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Western blot - SIRT1 antibody (ab28170)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- hSIR2 antibody
- hSIRT1 antibody
see all
Database links
- Entrez Gene: 93759 Mouse
- SwissProt: Q923E4 Mouse
- Unigene: 351459 Mouse
Anti-SIRT1 antibody images:
Western blot - SIRT1 antibody (ab28170)
Anti-SIRT1 antibody (ab28170) at 1 µg/ml + F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 110 kDa
Observed band size : 110 kDa
References for Anti-SIRT1 antibody (ab28170)
This product has been referenced in:
- Tarantino Cet al. miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells. FASEB J 24:3255-63 (2010). WB; Mouse.Read more (PubMed: 20439489) »
- Csiszar Aet al. Resveratrol induces mitochondrial biogenesis in endothelial cells. Am J Physiol Heart Circ Physiol 297:H13-20 (2009).Read more (PubMed: 19429820) »
See all 3 publications for this product
Publishing research using ab28170? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"