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ab7770 |
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ab7770 |
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Read our guarantee »Publishing research using ab7343? Please let us know so that we can cite the reference in this datasheet
ab7343 has been referenced in 7 publications.
Publishing research using ab7343? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 81.3 kDa
40ug of whole cell extract (over-expressing the SIRT1 protein) is probed with anti-SIRT1 antibody (ab7343).
The protein has not been detected in Western blot in HeLa lysates.
SIRT1 was immunoprecipitated from HeLa nuclear extract. The IP'd fraction was incubated with a histone H4 peptide acetylated with tritiated acetyl-coA, in the presence or absence of NAD+ (the cofactor for SIRT's). After incubation the free label (released acetyl groups) was separated from the peptide.
Paul Hurd
All lanes : Anti-SIRT1 antibody (ab7343) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Vector Transfected Control
Lane 2 : HEK293 Whole Cell Lysate Transiently Overexpressing SIRT1
Lysates/proteins at 5 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 81.3 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
ab7343 (1/200) staining SIRT1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
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