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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody
See all SIRT1 products (14) ...
Description
Rabbit polyclonal to SIRT1
Specificity
SIRT1
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SIRT1.
(Peptide available as ab7770.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 20385619)
IP: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 81.3 kDa.Can be blocked with SIRT1 peptide (ab7770).
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- hSIR2 antibody
- hSIRT1 antibody
see all
Database links
- Entrez Gene: 23411 Human
- Omim: 604479 Human
- SwissProt: Q96EB6 Human
- Unigene: 369779 Human
Anti-SIRT1 antibody images:
Western blot - Anti-SIRT1 antibody (ab7343)
Predicted band size : 81.3 kDa
40ug of whole cell extract (over-expressing the SIRT1 protein) is probed with anti-SIRT1 antibody (ab7343).
The protein has not been detected in Western blot in HeLa lysates.
Immunoprecipitation - Anti-SIRT1 antibody (ab7343)
SIRT1 was immunoprecipitated from HeLa nuclear extract. The IP'd fraction was incubated with a histone H4 peptide acetylated with tritiated acetyl-coA, in the presence or absence of NAD+ (the cofactor for SIRT's). After incubation the free label (released acetyl groups) was separated from the peptide.
Paul Hurd
Western blot - SIRT1 antibody (ab7343)
All lanes : Anti-SIRT1 antibody (ab7343) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Vector Transfected Control
Lane 2 : HEK293 Whole Cell Lysate Transiently Overexpressing SIRT1
Lysates/proteins at 5 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 81.3 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - SIRT1 antibody (ab7343)
ab7343 (1/200) staining SIRT1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
References for Anti-SIRT1 antibody (ab7343)
This product has been referenced in:
- Caito Set al. SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by oxidants and carbonyl stress. FASEB J 24:3145-59 (2010). WB, ICC/IF; Human.Read more (PubMed: 20385619) »
- Kornberg MDet al. GAPDH mediates nitrosylation of nuclear proteins. Nat Cell Biol 12:1094-100 (2010). IP; Human.Read more (PubMed: 20972425) »
See all 7 publications for this product
Publishing research using ab7343? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"