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Overview

  • Product nameSIRT1 protein (Active)See all SIRT1 proteins and peptides ...
  • Protein descriptionRecombinant fragment: Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193- 741, with an N-terminal enzyme tag.
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activitySpecific Activity: 8 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate, 200 uM NAD+ and 20 ng/ul Sirtuin 1. Incubation condition: 30 min at 30ºC.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 0.05% Tween 20, 50mM Tris, 2.7mM Potassium chloride, 138mM Sodium chloride, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab54334 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab123457,ab135392,ab135394,ab136807 and ab156585.

    • Protein info

    • Alternative names
        75SirT1BA57G10.4hSIR2
        hSIRT1NAD dependent deacetylase SIRT1NAD dependent deacetylase sirtuin 1NAD dependent protein deacetylase sirtuin 1OTTHUMP00000198111OTTHUMP00000198112Regulatory protein SIR2 homolog 1SIR1_HUMANSIR2 like 1SIR2 like protein 1SIR2-like protein 1SIR2ALPHASIR2alpha proteinSIR2L1SIRT 1Sirt1SIRT1 Sir2 like proteins (siruitins) type 1SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)SirtT1 75 kDa fragmentsirtuinsirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)Sirtuin 1Sirtuin type 1
      see all

    Target information above from: UniProt accession Q96EB6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for SIRT1 protein (Active) (ab54334)

    ab54334 has not yet been referenced specifically in any publications.

    Publishing research using ab54334 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"