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Product name
SIRT1 protein (Tagged)
See all SIRT1 products (7) ...
Protein description
Recombinant fragment: Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193- 741, with an N-terminal enzyme tag.
Expression host
E. coli
Properties
Purity
> 90% by SDS-PAGE
Biological activity
Specific Activity: 8 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate, 200 uM NAD+ and 20 ng/ul Sirtuin 1. Incubation condition: 30 min at 30ºC.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.05% Tween 20, 50mM Tris, 2.7mM Potassium chloride, 138mM Sodium chloride, pH 8.0
Concentration
Concentration information loading...
Research areas
Metabolism >> Types of disease >> Obesity
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Other Nuclear Bodies
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution.
Application notes
This peptide can be used with studies using ab123457.
Protein info
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for SIRT1 protein (Tagged) (ab54334)
ab54334 has not yet been referenced specifically in any publications.
Publishing research using ab54334? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"