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- Proteins and Peptides
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Product name
Anti-SIRT3 antibody
See all SIRT3 products (9) ...
Description
Goat polyclonal to SIRT3
Specificity
This antibody is expected to recognise both reported isoforms.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-QRETGKLDGPDK, corresponding to C terminal amino acids 246-257 of Human SIRT3 (NP_0017524.1 and same sequence for NP_036371.1)
C-QRETGKLDGPDK
Positive control
Human liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Cardiovascular >> Heart >> Cardiac metabolism
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - SIRT3 antibody (ab40006)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: 1/128000 -antibody detection limit dilution.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa). Can be blocked with SIRT3 peptide (246-257) (ab45830).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionQ9NTG7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hSIRT3 antibody
- hSIRT 3 antibody
- hSIRT3 antibody
see all
Database links
- Entrez Gene: 23410 Human
- Omim: 604481 Human
- SwissProt: Q9NTG7 Human
- Unigene: 716456 Human
Anti-SIRT3 antibody images:
Western blot - SIRT3 antibody (ab40006)
Anti-SIRT3 antibody (ab40006) at 0.2 µg/ml + Human Liver lysate in RIPA buffer at 35 µg
Predicted band size : 29 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
References for Anti-SIRT3 antibody (ab40006)
ab40006 has not yet been referenced specifically in any publications.
Publishing research using ab40006? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"