Anti-SIRT4 antibody (ab10140)
Key features and details
- Goat polyclonal to SIRT4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT4 antibody
See all SIRT4 primary antibodies -
Description
Goat polyclonal to SIRT4 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
SRCGELLPLIDPC
, corresponding to C terminal amino acids 302-314 of Human SIRT4. -
Positive control
- IHC: Human skeletal muscle and pancreas tissue WB: Human kidney and U937 lysate, HEK293 cell lysate
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General notes
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended.
|
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended. |
Target
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Function
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion. -
Tissue specificity
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. -
Sequence similarities
Belongs to the sirtuin family. Class II subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 519328 Cow
- Entrez Gene: 23409 Human
- Entrez Gene: 75387 Mouse
- Entrez Gene: 100125349 Pig
- Entrez Gene: 304539 Rat
- Omim: 604482 Human
- SwissProt: Q1JQC6 Cow
- SwissProt: Q9Y6E7 Human
see all -
Alternative names
- MGC130046 antibody
- MGC130047 antibody
- MGC57437 antibody
see all
Images
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Anti-SIRT4 antibody (ab10140) at 1 µg/ml + Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.
Predicted band size: 36 kDaab10140 staining (1
Incubation with the primary Ab was carried out for 1 hr at room temperature.µ g/ml) of Human Kidney lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab10140 has been referenced in 18 publications.
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Wang Y et al. SIRT4-Catalyzed Deacetylation of Axin1 Modulates the Wnt/β-Catenin Signaling Pathway. Front Oncol 12:872444 (2022). PubMed: 35707358
- Xu L et al. Circular RNA OMA1 regulates the progression of breast cancer via modulation of the miR-1276/SIRT4 axis. Mol Med Rep 24:N/A (2021). PubMed: 34414449
- Du L et al. Loss of SIRT4 promotes the self-renewal of Breast Cancer Stem Cells. Theranostics 10:9458-9476 (2020). PubMed: 32863939
- Wang T et al. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. Mol Cell 75:823-834.e5 (2019). PubMed: 31302001