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Anti-SIRT5 antibody [EPR5411] (ab108968)

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Overview

Product name

Anti-SIRT5 antibody [EPR5411]
See all SIRT5 products (6) ...

Description

Rabbit monoclonal [EPR5411] to SIRT5

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

Synthetic peptide corresponding to residues near the N terminus of Human SIRT5 (UniProt Q9NXA8).

Positive control

Fetal liver, HeLa and A431 cell lysates

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR5411

Isotype

IgG1

Research areas

Metabolism >> Types of disease >> Cancer
Cardiovascular >> Heart >> Cardiac metabolism
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - SIRT5 antibody [EPR5411] (ab108968)Western blot - SIRT5 antibody [EPR5411] (ab108968) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab108968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/10000. De...Read more →

    WB: 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Application notes

Is unsuitable for ,Flow Cyt,ICC,IHC-P or IP.

Target

Function

NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting. Deacetylates CPS1 and increases CPS1 activity in response to elevated NAD levels during fasting (By similarity). Can deacetylate cytochrome c and a number of other proteins (in vitro).

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.

Cellular localization

Mitochondrion matrix. Mitochondrion intermembrane space.

Target information above from: UniProt accessionQ9NXA8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • NAD dependent deacetylase sirtuin 5 antibody
  • NAD-dependent deacetylase sirtuin-5 antibody
  • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
  • Sir2 like 5 antibody
  • SIR2-like protein 5 antibody
  • SIR2L5 antibody
  • Sirt5 antibody
  • SIRT5_HUMAN antibody
  • Sirtuin type 5 antibody
see all

Database links

Anti-SIRT5 antibody [EPR5411] images:

  Western blot - SIRT5 antibody [EPR5411] (ab108968)

Western blot - SIRT5 antibody [EPR5411] (ab108968)

All lanes : Anti-SIRT5 antibody [EPR5411] (ab108968) at 1/1000 dilution

Lane 1 : Fetal liver cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit. at 1/2000 dilution

Predicted band size : 34 kDa
Observed band size : 34 kDa

References for Anti-SIRT5 antibody [EPR5411] (ab108968)

ab108968 has not yet been referenced specifically in any publications.

Publishing research using ab108968? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"