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Western blot - SIX1 antibody (ab86028)
(enlarge)
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Product Name
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SIX1 antibody
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See all SIX1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SIX1
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Immunogen
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Three synthetic peptides (human) conjugated to KLH
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 32 kDa). Tag is 14kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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An induced culture of E. coli
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
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Relevance
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The vertebrate SIX genes are homologs of the Drosophila 'sine oculis' (so) gene, which is expressed primarily in the developing visual system of the fly. Members of the SIX gene family encode proteins that are characterized by a divergent DNA-binding homeodomain and an upstream SIX domain, which may be involved both in determining DNA-binding specificity and in mediating protein protein interactions. Genes in the SIX family have been shown to play roles in vertebrate and insect development or have been implicated in maintenance of the differentiated state of tissues.
SIX1 may be involved in limb tendon and ligament development.
It is specifically expressed in skeletal muscle.
It belongs to the SIX/Sine oculis homeobox family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SIX1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SIX1 antibody images, references, products related to ab86028 and other tools.
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SIX1 antibody images:
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 Western blot - SIX1 antibody (ab86028)
All lanes : SIX1 antibody (ab86028) at 1/1000 dilution
Lane 1 : An un-induced culture of E. coli
Lane 2 : An induced culture of E. coli
Lysates/proteins at 1/5000 dilution per lane.
Secondary
Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 60 minutes
Gel concentration 10%
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Search PubMed (MEDLINE) for references to SIX1
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SIX1 antibody - more information
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Customer reviews (feedback) regarding SIX1 antibody |
Customer FAQs regarding SIX1 antibody |
Protocols for SIX1 antibody |
Price and availability of products related to SIX1 antibody |
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SIX1 antibody - related products:
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SIX1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SIX1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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