Anti-SLAM / CD150 antibody [9D1] - Low endotoxin, Azide free (ab174599)
Key features and details
- Rat monoclonal [9D1] to SLAM / CD150 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-SLAM / CD150 antibody [9D1] - Low endotoxin, Azide free
See all SLAM / CD150 primary antibodies -
Description
Rat monoclonal [9D1] to SLAM / CD150 - Low endotoxin, Azide free -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Mouse thymic and splenic cell suspensions (C57Bl/6 splenocytes).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2 -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9D1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.5-1µg for 105-8 cells.
(Final test volume = 100 µL). ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 0.5-1µg for 105-8 cells. (Final test volume = 100 µL). ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
Tissue specificity
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
Post-translational
modificationsPhosphorylated by FYN. -
Cellular localization
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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Database links
- Entrez Gene: 27218 Mouse
- SwissProt: Q9QUM4 Mouse
- Unigene: 103648 Mouse
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Alternative names
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab174599 has not yet been referenced specifically in any publications.