Anti-SLC16A3/MCT 4 antibody - C-terminal (ab190497)
Key features and details
- Rabbit polyclonal to SLC16A3/MCT 4 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC16A3/MCT 4 antibody - C-terminal
See all SLC16A3/MCT 4 primary antibodies -
Description
Rabbit polyclonal to SLC16A3/MCT 4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide within Human SLC16A3/MCT 4 aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001193879.1)
Database link: O15427 -
Positive control
- HeLa, Jurkat, 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
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Function
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. -
Tissue specificity
Highly expressed in skeletal muscle. -
Sequence similarities
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9123 Human
- Omim: 603877 Human
- SwissProt: O15427 Human
- Unigene: 500761 Human
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Form
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains. -
Alternative names
- MCT 4 antibody
- MCT3 antibody
- MCT4 antibody
see all
Images
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All lanes : Anti-SLC16A3/MCT 4 antibody - C-terminal (ab190497) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutes -
Immunoprecipitation analysis of HeLa cell lysate (1 mg per IP reaction; 20% of IP loaded) labeling SLC16A3/MCT 4 using ab190497 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated SLC16A3, ab190497 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190497 has not yet been referenced specifically in any publications.