Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC16A8
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A 14-22 aa region within synthetic peptide: FAVYAVTKFL MALGLFVPAI LLVNYAKDAG VPDTDAAFLL SIVGFVDIVA, corresponding to internal sequence amino acids 253-302 of Human SLC16A8

  • Positive control
    • Jurkat cell lysate (WB); Human kidney tissue (IHC-P).

Properties

Applications

Our Abpromise guarantee covers the use of ab60333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    SLC16A8 is proton-linked monocarboxylate transporter which catalyzes the rapid transport across the plasma membrane of monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, as well as the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • Cellular localization
    Membrane. Multi-pass membrane protein.
  • Database links
  • Alternative names
    • MCT 3 antibody
    • MCT3 antibody
    • Monocarboxylate transporter 3 antibody
    • Monocarboxylic acid transporter 3 antibody
    • OTTHUMP00000028510 antibody
    • REMP antibody
    • Solute carrier 16 (monocarboxylic acid transporters) member 8 antibody
    • Solute carrier family 16 member 8 antibody
    • solute carrier family 16, member 8 (monocarboxylic acid transporter 3) antibody
    see all

Images

  • Anti-SLC16A8 antibody (ab60333) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC26A8 with ab60333 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

This product has been referenced in:
  • Garnett JP  et al. Hyperglycaemia and Pseudomonas aeruginosa acidify cystic fibrosis airway surface liquid by elevating epithelial monocarboxylate transporter 2 dependent lactate-H(+) secretion. Sci Rep 6:37955 (2016). ICC/IF ; Human . Read more (PubMed: 27897253) »
  • Eilertsen M  et al. Monocarboxylate transporters 1-4 in NSCLC: MCT1 is an independent prognostic marker for survival. PLoS One 9:e105038 (2014). IHC-P ; Human . Read more (PubMed: 25225794) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab60333.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up