Anti-SLIRP antibody [1E10BC8] (ab119687)

  Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)

All lanes : Anti-SLIRP antibody [1E10BC8] (ab119687) at 1 µg/ml

Lane 1 : HepG2 lysate at 20 µg
Lane 2 : fibroblast lysate at 20 µg/ml
Lane 3 : SLIRP recombinant protein at 0.01 µg

Secondary
Infra red fluorescence - Goat anti-mouse 800 at 1/5000 dilution

Predicted band size : 12 kDa

  Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)

Immunocytochemistry of ab119687 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab119687, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.

  - Anti-SLIRP antibody [1E10BC8] (ab119687)

Fibroblast (HDFn) cells were stained with 1µg/mL SLIRP antibody (ab119687) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.