Anti-SLK antibody (ab226986)
Key features and details
- Rabbit polyclonal to SLK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLK antibody
See all SLK primary antibodies -
Description
Rabbit polyclonal to SLK -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SLK (internal sequence). The exact sequence is proprietary.
Database link: Q9H2G2 -
Positive control
- WB: A549 and H1299 whole cell extracts. ICC/IF: H1299 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 143 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 143 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 9748 Human
- SwissProt: Q9H2G2 Human
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Alternative names
- CTCL tumor antigen se20 9 antibody
- LOSK antibody
- se20 9 antibody
see all
Images
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All lanes : Anti-SLK antibody (ab226986) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : SLK Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 143 kDa -
4% paraformaldehyde-fixed H1299 cells stained for SLK (green) using ab226986 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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All lanes : Anti-SLK antibody (ab226986) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : H1299 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 143 kDa5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226986 has not yet been referenced specifically in any publications.