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- Secondary antibodies
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Product name
Anti-SLUG antibody
See all SLUG products (8) ...
Description
Rabbit polyclonal to SLUG
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide selected from the N-terminal region of human SLUG conjugated to KLH.
Positive control
A2058 cell line lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab75629 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Nervous system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Stem Cells >> Lineage Markers >> Ectoderm
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Western blot - SLUG antibody (ab75629)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ELISA
ELISA: 1/1000.ELISA: 1/1000.
Target
Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells.
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO43623
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Omim: 602150 Human
- SwissProt: O43623 Human
- SwissProt: Q3UZ96 Mouse
- SwissProt: P97469 Mouse
- Unigene: 360174 Human
- Unigene: 4272 Mouse
Anti-SLUG antibody images:
Western blot - SLUG antibody (ab75629)
Anti-SLUG antibody (ab75629) at 1/50 dilution + A2058 cell line lysates at 35 µg
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 85 kDa,87 kDa. We are unsure as to the identity of these extra bands.
References for Anti-SLUG antibody (ab75629)
This product has been referenced in:
- Wang FE et al. MicroRNA-204/211 alters epithelial physiology. FASEB J : (2010).Read more (PubMed: 20056717) »
See 1 publication for this product
Publishing research using ab75629? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"