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Anti-SMAD 1/5/8/9 antibody (ab13723)

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Overview

  • Product nameAnti-SMAD 1/5/8/9 antibody
  • Description
    Rabbit polyclonal to SMAD 1/5/8/9
  • Specificityab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 18-32 and amino acids 315-329 of Human SMAD1. These sequences are identical in human SMAD5 and SMAD8/9.

  • Positive controlA549 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa.

Target

  • RelevanceSMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.
  • Cellular localizationCytoplasmic
  • Database links
    see all
  • Alternative names
      DPC4 antibodyMadh6 antibodyMADH9 antibody
      MADR1 antibodySMAD family member 1 antibodySMAD family member 5 antibodySMAD family member 9 antibodySMAD8A antibodySMAD8B antibody
    see all

Anti-SMAD 1/5/8/9 antibody images

  • Western blot - SMAD 1/5/8/9 antibody (ab13723)
    Western blot - SMAD 1/5/8/9 antibody (ab13723)
    Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.

References for Anti-SMAD 1/5/8/9 antibody (ab13723)

ab13723 has not yet been referenced specifically in any publications.

Publishing research using ab13723 ? Please let us know so that we can cite the reference in this datasheet

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Can you please send me a list of the antibodies you have available for total SMAD 1/5 and phospho S463 / S465? I am looking for two antibodies to use in WB on the LICOR Odyssey system, so they should be raised in different species.

Thank you for contacting us.

We currently have two rabbit polyclonal antibodies that will detect SMAD 1/5:

Rabbit polyclonal to SMAD1/5/9 - ab80255
Rabbit polyclonal to SMAD 1/5/8/9 - ab13723

We have a number of antibodi...

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What is the difference between Smad 8 and 9. Are they different proteins?

Thank you for your enquiry. Following some searching of the relevant databases it seems that Smad8 is the previous name of Smad9. Please see the following references: http://marvester.embl.de/marvester/Q9JI/Q9JIW5.htm http://nsm1.utdallas.edu/bio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"