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RabMAbs (3576-1)

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)

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Overview

  • Product nameAnti-SMAD 1 + SMAD9 antibody [EPR2290(2)]
  • Description
    Rabbit monoclonal [EPR2290(2)] to SMAD 1 + SMAD9
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SMAD 1 + SMAD9.

  • Positive control293T, A673, Human heart and HeLa lysates
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab108965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • Application notesIs unsuitable for ,Flow Cyt,ICC,IHC-P or IP.

    • Target

    • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similaritiesBelongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

      • Target information above from: UniProt accession Q15797 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        BSP-1 antibodyBSP1 antibodyHsMAD1 antibody
        JV4-1 antibodyMAD homolog 1 antibodyMad-related protein 1 antibodyMADH1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySMAD 1 antibodySMAD family member 1 antibodySmad1 antibodySMAD1_HUMAN antibodyTransforming growth factor-beta-signaling protein 1 antibody
      see all

    Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] images

    • Western blot - SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)
      Western blot - SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)
      All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : A673 lysate
      Lane 3 : Human heart lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1, 3 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution


      Predicted band size : 52 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)

    References for Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)

    ab108965 has not yet been referenced specifically in any publications.

    Publishing research using ab108965 ? Please let us know so that we can cite the reference in this datasheet

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    Looking for a good total Smad Antibody for WB in mouse

    We carry products which recognize mouse SMAD1/9, SMAD2/3 or SMAD1/5/9. There is no antibody currently on our catalog which recognizes all forms of SMAD in mouse. I have added links to our anti- SMAD1/9, SMAD2/3 and SMAD1/5/9 antibodies for your review bel...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"