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Overview

  • Product nameAnti-SMARCD3 antibodySee all SMARCD3 primary antibodies ...
  • Description
    Rabbit polyclonal to SMARCD3
  • SpecificityThis antibody reacts with SMARCD3
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: FERKLDQTIM RKRVDIQEAL KRPMKQKRKL RLYISNTFNP AKPDAEDSDG, corresponding to amino acids 128-177 of Human SMARCD3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control293T cell lysate

Applications

Our Abpromise guarantee covers the use of ab50556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA ELISA: 1/1.5625e+006.

Target

  • FunctionPlays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificityIsoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
  • Sequence similaritiesBelongs to the SMARCD family.
    Contains 1 SWIB domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q6STE5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      60 kDa BRG 1/Brm associated factor subunit C antibody60 kDa BRG-1/Brm-associated factor subunit C antibodyBAF60C antibody
      BRG1 associated factor 60C antibodyBRG1-associated factor 60C antibodyCRACD3 antibodyMGC111010 antibodyRsc6p antibodySmarcd3 antibodySMRD3_HUMAN antibodySWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3 antibodySWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 antibody
    see all

Anti-SMARCD3 antibody images

  • Western blot - SMARCD3 antibody (ab50556)
    Western blot - SMARCD3 antibody (ab50556)
    Lane 1 :
    Lane 2 : Anti-SMARCD3 antibody (ab50556) at 0.5 µg/ml

    Lane 1 : MARKER
    Lane 2 : 293T cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 55 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

References for Anti-SMARCD3 antibody (ab50556)

ab50556 has not yet been referenced specifically in any publications.

Publishing research using ab50556 ? Please let us know so that we can cite the reference in this datasheet

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