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Overview

  • Product nameAnti-SMC1 antibody [C2M]See all SMC1 primary antibodies ...
  • Description
    Mouse monoclonal [C2M] to SMC1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 402-894 of Human SMC1.

  • Positive controlHeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 143 kDa).

    Several customers have reported the antibody not working well in IHC. We have also had reports that this antibody does not work in ICC/IF on HeLa cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Alternative names
        bK268H5 antibodybK268H5.5 antibodyCDLS2 antibody
        DKFZp686L19178 antibodyDKFZp686L19178 antibodyDXhXs423e antibodyDXS423E antibodyDXS423E antibodyFLJ43748 antibodyKIAA0178 antibodyMGC138332 antibodyMGC138332 antibodyOTTHUMP00000061876 antibodyRP6 29D12.1 antibodySb1.8 antibodySB1.8 antibodySB1.8/DXS423E protein antibodySegregation of mitotic chromosomes 1 antibodySegregation of mitotic chromosomes like 1 antibodySMC 1 antibodySMC protein 1B antibodySMC-1-beta antibodySMC-1B antibodySMC1 antibodySMC1A antibodySMC1alpha antibodySMC1alpha protein antibodySMC1B antibodySMC1B_HUMAN antibodySMC1BETA antibodySMC1beta protein antibodySMC1L1 antibodySMC1L2 antibodySMCB antibodyStructural maintenance of chromosome 1 like 1 protein antibodyStructural maintenance of chromosome 1 like 2 protein antibodystructural maintenance of chromosomes 1-like 2 (yeast) antibodyStructural maintenance of chromosomes 1A antibodyStructural maintenance of chromosomes 1B antibodyStructural maintenance of chromosomes protein 1B antibodyStructural Maintenance of Chromosomes-1 Like 1 antibody
      see all

    Anti-SMC1 antibody [C2M] images

    • Western blot - SMC1 antibody [C2M] (ab16147)
      Western blot - SMC1 antibody [C2M] (ab16147)
      All lanes : Anti-SMC1 antibody [C2M] (ab16147) at 1/1000 dilution

      Lane 1 : Human BT-20 (breast cancer cell line) - whole cell lysate
      Lane 2 : Human BT-474 (breast cancer cell line) - whole cell lysate
      Lane 3 : Human HBL-100 (breast cancer cell line) - whole cell lysate
      Lane 4 : Human HCC-1937 (breast cancer cell line) - whole cell lysate
      Lane 5 : Human HME-1 (breast cancer cell line) - whole cell lysate
      Lane 6 : Human Hs-578T (breast cancer cell line) - whole cell lysate
      Lane 7 : Human MCF-10A (breast cancer cell line) - whole cell lysate
      Lane 8 : Human MDA-MB 157(breast cancer cell line) - whole cell lysate
      Lane 9 : Human MDA-MB 231 (breast cancer cell line) - whole cell lysate
      Lane 10 : Human MDA-MB 436(breast cancer cell line) - whole cell lysate
      Lane 11 : Human MDA-MB 453 (breast cancer cell line) - whole cell lysate
      Lane 12 : Human MDA-MB 549 (breast cancer cell line) - whole cell lysate
      Lane 13 : Human T47D (breast cancer cell line) - whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      HRP conjugated rabbit anti-mouse at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 143 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 6 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-SMC1 antibody [C2M] (ab16147)

    ab16147 has not yet been referenced specifically in any publications.

    Publishing research using ab16147 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-SMC1 antibody [C2M]

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount 50 µg
    Specification breast cancer cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Jul 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"