Overview

  • Product nameAnti-SMC1 antibody [C2M]See all SMC1 primary antibodies ...
  • Description
    Mouse monoclonal [C2M] to SMC1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 402-894 of Human SMC1.

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 143 kDa).

    Several customers have reported the antibody not working well in IHC. We have also had reports that this antibody does not work in ICC/IF on HeLa cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • bK268H5 antibody
      • bK268H5.5 antibody
      • CDLS2 antibody
      • DKFZp686L19178 antibody
      • DKFZp686L19178 antibody
      • DXhXs423e antibody
      • DXS423E antibody
      • DXS423E antibody
      • FLJ43748 antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • MGC138332 antibody
      • OTTHUMP00000061876 antibody
      • RP6 29D12.1 antibody
      • Sb1.8 antibody
      • SB1.8 antibody
      • SB1.8/DXS423E protein antibody
      • Segregation of mitotic chromosomes 1 antibody
      • Segregation of mitotic chromosomes like 1 antibody
      • SMC 1 antibody
      • SMC protein 1B antibody
      • SMC-1-beta antibody
      • SMC-1B antibody
      • SMC1 antibody
      • SMC1A antibody
      • SMC1alpha antibody
      • SMC1alpha protein antibody
      • SMC1B antibody
      • SMC1B_HUMAN antibody
      • SMC1BETA antibody
      • SMC1beta protein antibody
      • SMC1L1 antibody
      • SMC1L2 antibody
      • SMCB antibody
      • Structural maintenance of chromosome 1 like 1 protein antibody
      • Structural maintenance of chromosome 1 like 2 protein antibody
      • structural maintenance of chromosomes 1-like 2 (yeast) antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes 1B antibody
      • Structural maintenance of chromosomes protein 1B antibody
      • Structural Maintenance of Chromosomes-1 Like 1 antibody
      see all

    Anti-SMC1 antibody [C2M] images

    • All lanes : Anti-SMC1 antibody [C2M] (ab16147) at 1/1000 dilution

      Lane 1 : Human BT-20 (breast cancer cell line) - whole cell lysate
      Lane 2 : Human BT-474 (breast cancer cell line) - whole cell lysate
      Lane 3 : Human HBL-100 (breast cancer cell line) - whole cell lysate
      Lane 4 : Human HCC-1937 (breast cancer cell line) - whole cell lysate
      Lane 5 : Human HME-1 (breast cancer cell line) - whole cell lysate
      Lane 6 : Human Hs-578T (breast cancer cell line) - whole cell lysate
      Lane 7 : Human MCF-10A (breast cancer cell line) - whole cell lysate
      Lane 8 : Human MDA-MB 157(breast cancer cell line) - whole cell lysate
      Lane 9 : Human MDA-MB 231 (breast cancer cell line) - whole cell lysate
      Lane 10 : Human MDA-MB 436(breast cancer cell line) - whole cell lysate
      Lane 11 : Human MDA-MB 453 (breast cancer cell line) - whole cell lysate
      Lane 12 : Human MDA-MB 549 (breast cancer cell line) - whole cell lysate
      Lane 13 : Human T47D (breast cancer cell line) - whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      HRP conjugated rabbit anti-mouse at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 143 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 6 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-SMC1 antibody [C2M] (ab16147)

    ab16147 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount 50 µg
    Specification breast cancer cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"