Overview

  • Product nameAnti-SMC1 antibodySee all SMC1 primary antibodies ...
  • Description
    Rabbit polyclonal to SMC1
  • Tested applicationsICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human SMC1.

    (Peptide available as ab23863.)

  • Positive control
    • HeLa and Jurkat whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab21583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).Can be blocked with Human SMC1 peptide (ab23863).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • bK268H5 antibody
    • bK268H5.5 antibody
    • CDLS2 antibody
    • DKFZp686L19178 antibody
    • DKFZp686L19178 antibody
    • DXhXs423e antibody
    • DXS423E antibody
    • DXS423E antibody
    • FLJ43748 antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • MGC138332 antibody
    • OTTHUMP00000061876 antibody
    • RP6 29D12.1 antibody
    • Sb1.8 antibody
    • Sb1.8 antibody
    • SB1.8 antibody
    • SB1.8/DXS423E protein antibody
    • SB1.8/DXS423E protein antibody
    • Segregation of mitotic chromosomes 1 antibody
    • Segregation of mitotic chromosomes like 1 antibody
    • SMC 1 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 antibody
    • SMC1 antibody
    • SMC1A antibody
    • SMC1alpha antibody
    • SMC1alpha protein antibody
    • SMC1alpha protein antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1BETA antibody
    • SMC1beta protein antibody
    • SMC1L1 antibody
    • SMC1L1 antibody
    • SMC1L2 antibody
    • SMC1L2 antibody
    • SMCB antibody
    • Structural maintenance of chromosome 1 like 1 protein antibody
    • Structural maintenance of chromosome 1 like 2 protein antibody
    • structural maintenance of chromosomes 1-like 2 (yeast) antibody
    • structural maintenance of chromosomes 1-like 2 (yeast) antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    • Structural Maintenance of Chromosomes-1 Like 1 antibody
    • Structural Maintenance of Chromosomes-1 Like 1 antibody
    see all

Anti-SMC1 antibody images

  • All lanes : Anti-SMC1 antibody (ab21583) at 1 µg/ml

    Lane 1 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg
    Lane 3 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SMC1 peptide (ab23863) at 1 µg/ml
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg with Human SMC1 peptide (ab23863) at 1 µg/ml

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 143 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • SMC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SMC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21583.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 150kDa: SMC1; Non specific - 41 and 42kDa: We are unsure as to the identity of this extra band.
  • ICC/IF image of ab21583 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21583, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    Panel A shows localisation of ab21583 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.

  • ab21583 staining SMC1 in assynchonous HeLa cells (green). Cells were paraformaldehyde-fixed (4% - 10min) and counterstained with DAPI (red). Secondary antibody: Goat anti-Rabbit conjugated to Cy3 ®. Please refer to Abreview for further details.

    See Abreview

  • IHC image of SMC1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21583, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-SMC1 antibody (ab21583)

This product has been referenced in:
  • Parenti I  et al. Overall and allele-specific expression of the SMC1A gene in female Cornelia de Lange syndrome patients and healthy controls. Epigenetics 9:N/A (2014). WB . Read more (PubMed: 24756084) »
  • Liu B  et al. Proteomic identification of common SCF ubiquitin ligase FBXO6-interacting glycoproteins in three kinds of cells. J Proteome Res 11:1773-81 (2012). WB . Read more (PubMed: 22268729) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse stem cell)
Specification mouse stem cell
Fixative Formaldehyde
Permeabilization Yes - triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Sep 02 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"