Anti-SMC1 antibody - ChIP Grade (ab9262)

Western blot - SMC1 antibody (ab9262)

ab9262 at a 1:3000 dilution staining SMC 1 in 100µg of HeLa nuclear extract by western Blot (ECL).

ab9262 at a 1:3000 dilution staining SMC 1 in 100µg of HeLa nuclear extract by western Blot (ECL).

Immunocytochemistry - SMC1 antibody (ab9262)

Interphase HeLa cells stained with ab9262 (1/500). The antibody gave nuclear staining in all interphase nuclei investigated. However,  the antibody failed to recognize any chromatin-associated epitopes in prophase or metaphase cells suggesting that the epitopes may be masked during mitosis. ab9262 staining is shown in green. The cells were counterstained with DAPI (red). 100x magnification.

Kirk McManus, University of British Columbia

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SMC1 antibody(ab9262)

Ab9262 staining human normal colon tissue. Staining is localised to nuclear compartment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.