RabMAb

Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)

Overview

  • Product nameAnti-SMC1 (phospho S957) antibody [EPR2857Y]
    See all SMC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2857Y] to SMC1 (phospho S957)
  • SpecificityDetects SMC1 only when phosphorylated on serine 957.
  • Tested applicationsWB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-peptide corresponding to residues surrounding serine 957 of human SMC1

  • Positive control
    • Hela cell lysate; human breast carcinoma or tonsil tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 143 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • CDLS2 antibody
      • DKFZp686L19178 antibody
      • DXhXs423e antibody
      • DXS423E antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • OTTHUMP00000061876 antibody
      • RP6 29D12.1 antibody
      • SB1.8 antibody
      • Segregation of mitotic chromosomes 1 antibody
      • Segregation of mitotic chromosomes like 1 antibody
      • SMC 1 antibody
      • SMC protein 1B antibody
      • SMC-1-beta antibody
      • SMC-1B antibody
      • SMC1 antibody
      • SMC1A antibody
      • SMC1alpha antibody
      • SMC1alpha protein antibody
      • SMC1B antibody
      • SMC1B_HUMAN antibody
      • SMC1BETA antibody
      • SMC1beta protein antibody
      • SMC1L1 antibody
      • SMC1L2 antibody
      • SMCB antibody
      • Structural maintenance of chromosome 1 like 1 protein antibody
      • Structural maintenance of chromosome 1 like 2 protein antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes 1B antibody
      • Structural maintenance of chromosomes protein 1B antibody
      see all

    Anti-SMC1 (phospho S957) antibody [EPR2857Y] images

    • All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution

      Lane 1 : Hela cells lysate, untreated
      Lane 2 : Hela cells lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 143 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • ab75768 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
    • ab75768 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.

    References for Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)

    ab75768 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"