Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)

Overview

• Product nameAnti-SMC1 (phospho S957) antibody [EPR2857Y]See all SMC1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR2857Y] to SMC1 (phospho S957)
• SpecificityDetects SMC1 only when phosphorylated on serine 957.
• Tested applicationsWB, IHC-P, ICC more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A phospho-peptide corresponding to residues surrounding serine 957 of human SMC1

• Positive controlHela cell lysate; human breast carcinoma or tonsil tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR2857Y
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Cohesins

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

Target information above from: UniProt accession Q8NDV3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8243 Human
  • Entrez Gene: 27127 Human
  • Entrez Gene: 27127 Human
  • Omim: 608685 Human
  • Omim: 300040 Human
  • SwissProt: Q14683 Human
  • SwissProt: Q8NDV3 Human
  • SwissProt: Q8NDV3 Human

  • Unigene: 334176 Human

  see all

• Alternative names
  CDLS2 antibodyDKFZp686L19178 antibodyDXhXs423e antibody

  DXS423E antibodyKIAA0178 antibodyMGC138332 antibodyOTTHUMP00000061876 antibodyRP6 29D12.1 antibodySB1.8 antibodySegregation of mitotic chromosomes 1 antibodySegregation of mitotic chromosomes like 1 antibodySMC 1 antibodySMC protein 1B antibodySMC-1-beta antibodySMC-1B antibodySMC1 antibodySMC1A antibodySMC1alpha antibodySMC1alpha protein antibodySMC1B antibodySMC1B_HUMAN antibodySMC1BETA antibodySMC1beta protein antibodySMC1L1 antibodySMC1L2 antibodySMCB antibodyStructural maintenance of chromosome 1 like 1 protein antibodyStructural maintenance of chromosome 1 like 2 protein antibodyStructural maintenance of chromosomes 1A antibodyStructural maintenance of chromosomes 1B antibodyStructural maintenance of chromosomes protein 1B antibody

  see all

Anti-SMC1 (phospho S957) antibody [EPR2857Y] images

• 

  Western blot - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)
  All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution
  
  Lane 1 : Hela cells lysate, untreated
  Lane 2 : Hela cells lysate, treated with AP
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-conjugated goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 143 kDa
  Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)
  ab75768 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)
  ab75768 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.