Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)
- Product nameAnti-SMC1 (phospho S957) antibody [EPR2857Y]See all SMC1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR2857Y] to SMC1 (phospho S957)
- SpecificityDetects SMC1 only when phosphorylated on serine 957.
- Tested applicationsWB, IHC-P, ICC more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
A phospho-peptide corresponding to residues surrounding serine 957 of human SMC1
- Positive controlHela cell lysate; human breast carcinoma or tonsil tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR2857Y
- Research Areas
Our Abpromise guarantee covers the use of ab75768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Predicted molecular weight: 143 kDa.|
|IHC-P||IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC||ICC: 1/100 - 1/250.|
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Anti-SMC1 (phospho S957) antibody [EPR2857Y] images
All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution
Lane 1 : Hela cells lysate, untreated
Lane 2 : Hela cells lysate, treated with AP
Lysates/proteins at 10 µg per lane.
HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size : 143 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
ab75768 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
ab75768 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.
References for Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)
ab75768 has not yet been referenced specifically in any publications.