Anti-SMC1 (phospho S966) antibody [EP2858Y] (ab81306)

Overview

• Product nameAnti-SMC1 (phospho S966) antibody [EP2858Y]See all SMC1 primary antibodies ...
• Description
  Rabbit monoclonal [EP2858Y] to SMC1 (phospho S966)
• SpecificityThis antibody detects SMC1 phosphorylated on Serine 966.
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A phospho specific peptide corresponding to residues surrounding serine 966 of Human SMC1.

• Positive controlHeLa cell lysates. HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP2858Y
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Cohesins

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

Target information above from: UniProt accession Q8NDV3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8243 Human
  • Entrez Gene: 27127 Human
  • Entrez Gene: 27127 Human
  • Omim: 608685 Human
  • Omim: 300040 Human
  • SwissProt: Q14683 Human
  • SwissProt: Q8NDV3 Human
  • SwissProt: Q8NDV3 Human

  • Unigene: 334176 Human

  see all

• Alternative names
  CDLS2 antibodyDKFZp686L19178 antibodyDXhXs423e antibody

  DXS423E antibodyKIAA0178 antibodyMGC138332 antibodyOTTHUMP00000061876 antibodyRP6 29D12.1 antibodyRP6-29D12.1 antibodySB1.8 antibodysegregation of mitotic chromosomes 1 antibodysegregation of mitotic chromosomes like 1 antibodySMC 1 antibodySMC protein 1B antibodySMC-1-beta antibodySMC-1B antibodySMC1 antibodySMC1A antibodySMC1alpha antibodySMC1alpha protein antibodySMC1B antibodySMC1B_HUMAN antibodySMC1BETA antibodySMC1beta protein antibodySMC1L1 antibodySMC1L2 antibodySMCB antibodyStructural maintenance of chromosome 1 like 1 protein antibodyStructural maintenance of chromosome 1 like 2 protein antibodystructural maintenance of chromosomes 1A antibodyStructural maintenance of chromosomes 1B antibodyStructural maintenance of chromosomes protein 1B antibody

  see all

Anti-SMC1 (phospho S966) antibody [EP2858Y] images

• 

  Western blot - SMC1 (phospho S966) antibody [EP2858Y] (ab81306)
  All lanes : Anti-SMC1 (phospho S966) antibody [EP2858Y] (ab81306) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysates un-treated
  Lane 2 : HeLa cell lysates treated with hydroxyurea
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 143 kDa
  Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - SMC1 (phospho S966) antibody [EP2858Y] (ab81306)
  Immunofluorescent staining of HeLa cells using ab81306 at a 1/250 dilution.