Anti-SMC1 (phospho S966) antibody [EP2858Y] (ab81306)
- Product nameAnti-SMC1 (phospho S966) antibody [EP2858Y]See all SMC1 primary antibodies ...
- DescriptionRabbit monoclonal [EP2858Y] to SMC1 (phospho S966)
- SpecificityThis antibody detects SMC1 phosphorylated on Serine 966.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
A phospho specific peptide corresponding to residues surrounding serine 966 of Human SMC1.
- Positive control
- HeLa cell lysates. HeLa cells.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEP2858Y
- Research Areas
Our Abpromise guarantee covers the use of ab81306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/5000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).|
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Anti-SMC1 (phospho S966) antibody [EP2858Y] images
All lanes : Anti-SMC1 (phospho S966) antibody [EP2858Y] (ab81306) at 1/1000 dilution
Lane 1 : HeLa cell lysates un-treated
Lane 2 : HeLa cell lysates treated with hydroxyurea
Lysates/proteins at 10 µg per lane.
HRP goat anti-rabbit at 1/2000 dilution
Predicted band size : 143 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Immunofluorescent staining of HeLa cells using ab81306 at a 1/250 dilution.
References for Anti-SMC1 (phospho S966) antibody [EP2858Y] (ab81306)
ab81306 has not yet been referenced specifically in any publications.