Overview

  • Product nameAnti-SMC1 (phospho S966) antibodySee all SMC1 primary antibodies ...
  • Description
    Rabbit polyclonal to SMC1 (phospho S966)
  • Tested applicationsIHC-P, IP, WB, ICC/IF, ICC more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Orangutan
  • Immunogen

    Rabbits were immunized with a phosphorylated synthetic peptide, which represented the portion of human Structural Maintenance of Chromosomes-1 (LocusLink ID 8243)around Serine 966, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.

  • Positive control
    • HeLa cells treated with 10 Gy IR for 1hr. IHC-P: FFPE human spleen tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab1276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 2 - 6 µg/ml.
WB 1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Target information above from: UniProt accession Q8NDV3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • bK268H5 antibody
    • bK268H5.5 antibody
    • CDLS2 antibody
    • DKFZp686L19178 antibody
    • DKFZp686L19178 antibody
    • DXhXs423e antibody
    • DXS423E antibody
    • DXS423E antibody
    • FLJ43748 antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • MGC138332 antibody
    • OTTHUMP00000061876 antibody
    • RP6 29D12.1 antibody
    • Sb1.8 antibody
    • SB1.8 antibody
    • SB1.8/DXS423E protein antibody
    • Segregation of mitotic chromosomes 1 antibody
    • Segregation of mitotic chromosomes like 1 antibody
    • SMC 1 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 antibody
    • SMC1 antibody
    • SMC1A antibody
    • SMC1alpha antibody
    • SMC1alpha protein antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1BETA antibody
    • SMC1beta protein antibody
    • SMC1L1 antibody
    • SMC1L2 antibody
    • SMCB antibody
    • Structural maintenance of chromosome 1 like 1 protein antibody
    • Structural maintenance of chromosome 1 like 2 protein antibody
    • structural maintenance of chromosomes 1-like 2 (yeast) antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    • Structural Maintenance of Chromosomes-1 Like 1 antibody
    see all

Anti-SMC1 (phospho S966) antibody images

  • IHC image of SMC1 (phospho S966)  staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1276, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 143 kDa
    ab1276 at a 1:1000 dilution staining phospho SMC 1 (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL). ab1276 at a 1:1000 dilution staining phospho SMC 1 (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL).
  • SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1 (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).

References for Anti-SMC1 (phospho S966) antibody (ab1276)

This product has been referenced in:
  • Valdez BC  et al. The synergistic cytotoxicity of clofarabine, fludarabine and busulfan in AML cells involves ATM pathway activation and chromatin remodeling. Biochem Pharmacol 81:222-32 (2011). WB ; Human . Read more (PubMed: 20933509) »
  • Larsen DH  et al. The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage. J Cell Biol 190:731-40 (2010). WB ; Human . Read more (PubMed: 20805324) »

See all 10 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm that the immunogen used to generate ab1276 SMC1 (phospho S966) antibody represents a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Treatment X radiation (10g)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Mar 28 2007

Application Immunocytochemistry
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"