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- Secondary antibodies
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Product name
Anti-SMC1 (phospho S966) antibody
See all SMC1 products (15) ...
Description
Rabbit polyclonal to SMC1 (phospho S966)
Tested applications
IP, WB, IF, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Rabbits were immunized with a phosphorylated synthetic peptide, which represented the portion of human Structural Maintenance of Chromosomes-1 (LocusLink ID 8243)around Serine 966, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.
Positive control
HeLa cells treated with 10 Gy IR for 1hr.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for SMC1 pSer957 were affinity purified using the phosphopeptide immobilized onsolid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Cohesins
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 2 - 6 µg/ml.
WB: 1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
IF: 1/200. This application was successful in SKN cells at the recommended dilution (see images below), however the staining appeared associated with the cytoskeleton in Hela cells (data not shown).
ICC: Use at an assay dependent dilution.
Target
Relevance
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes and can lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion complex associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo.
Cellular localization
Nuclear
Alternative names
- CDLS2 antibody
- DKFZp686L19178 antibody
- DXS423E antibody
see all
Database links
- Entrez Gene: 27127 Human
- Entrez Gene: 8243 Human
- Omim: 608685 Human
- Omim: 300040 Human
- SwissProt: Q14683 Human
- SwissProt: Q8NDV3 Human
- Unigene: 334176 Human
Anti-SMC1 (phospho S966) antibody images:
Western blot - SMC1 (phospho S966) antibody (ab1276)
Predicted band size : 143 kDa
ab1276 at a 1:1000 dilution staining phospho SMC 1 (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL).
Immunocytochemistry - SMC1 (phospho S966) antibody (ab1276)
SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1 (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).
This image was submitted as part of a review by Kirk McManus, University of British Columbia
References for Anti-SMC1 (phospho S966) antibody (ab1276)
This product has been referenced in:
- Goldberg M et al. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 421:952-6 (2003).Read more (PubMed: 12607003) »
- Yazdi PT et al. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev 16:571-82 (2002).Read more (PubMed: 11877377) »
See all 10 publications for this product
Publishing research using ab1276? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"