Anti-SMC1 (phospho S966) antibody (ab73994)

Overview

  • Product nameAnti-SMC1 (phospho S966) antibodySee all SMC1 primary antibodies ...
  • Description
    Rabbit polyclonal to SMC1 (phospho S966)
  • Specificityab73994 detects SMC1 only when phosphorylated at serine 966.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phospho-peptide derived from human SMC1 around the phosphorylation site of serine 966 (S-G-SP-Q-R).

  • Positive control
    • human lung carcinoma tissue, extracts from HUVEC cells, treated with etoposide (24uM, 24hours)

Properties

Applications

Our Abpromise guarantee covers the use of ab73994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • CDLS2 antibody
    • DKFZp686L19178 antibody
    • DXhXs423e antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • OTTHUMP00000061876 antibody
    • RP6 29D12.1 antibody
    • SB1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • Segregation of mitotic chromosomes like 1 antibody
    • SMC 1 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 antibody
    • SMC1A antibody
    • SMC1alpha antibody
    • SMC1alpha protein antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1BETA antibody
    • SMC1beta protein antibody
    • SMC1L1 antibody
    • SMC1L2 antibody
    • SMCB antibody
    • Structural maintenance of chromosome 1 like 1 protein antibody
    • Structural maintenance of chromosome 1 like 2 protein antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    see all

Anti-SMC1 (phospho S966) antibody images

  • All lanes : Anti-SMC1 (phospho S966) antibody (ab73994) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells treated with etoposide (24uM, 24hours),
    Lane 2 : extracts from HUVEC cells, treated with etoposide (24uM, 24hours), with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 143 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <72 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using 1/50 ab73994. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

References for Anti-SMC1 (phospho S966) antibody (ab73994)

ab73994 has not yet been referenced specifically in any publications.

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