Anti-SMC1 (phospho S966) antibody (ab73994)
- Product nameAnti-SMC1 (phospho S966) antibodySee all SMC1 primary antibodies ...
- DescriptionRabbit polyclonal to SMC1 (phospho S966)
- Specificityab73994 detects SMC1 only when phosphorylated at serine 966.
- Tested applicationsWB, ELISA, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phospho-peptide derived from human SMC1 around the phosphorylation site of serine 966 (S-G-SP-Q-R).
- Positive control
- human lung carcinoma tissue, extracts from HUVEC cells, treated with etoposide (24uM, 24hours)
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab73994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).|
|IHC-P||IHC-P: 1/50 - 1/100.|
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Anti-SMC1 (phospho S966) antibody images
All lanes : Anti-SMC1 (phospho S966) antibody (ab73994) at 1/500 dilution
Lane 1 : extracts from HUVEC cells treated with etoposide (24uM, 24hours),
Lane 2 : extracts from HUVEC cells, treated with etoposide (24uM, 24hours), with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 143 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Additional bands at : <72 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using 1/50 ab73994. Left: untreated sample. Right: sample treated with immunising phosphopeptide.
References for Anti-SMC1 (phospho S966) antibody (ab73994)
ab73994 has not yet been referenced specifically in any publications.