Anti-SMC1 (phospho S966) antibody (ab73994)

Overview

• Product nameAnti-SMC1 (phospho S966) antibodySee all SMC1 primary antibodies ...
• Description
  Rabbit polyclonal to SMC1 (phospho S966)
• Specificityab73994 detects SMC1 only when phosphorylated at serine 966.
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phospho-peptide derived from human SMC1 around the phosphorylation site of serine 966 (S-G-S^P-Q-R).

• Positive controlhuman lung carcinoma tissue, extracts from HUVEC cells, treated with etoposide (24uM, 24hours)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Cohesins

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

Target information above from: UniProt accession Q8NDV3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8243 Human
  • Entrez Gene: 27127 Human
  • Entrez Gene: 27127 Human
  • Entrez Gene: 24061 Mouse
  • Entrez Gene: 140557 Mouse
  • Omim: 608685 Human
  • Omim: 300040 Human
  • SwissProt: Q14683 Human

  • SwissProt: Q8NDV3 Human
  • SwissProt: Q8NDV3 Human
  • SwissProt: Q9CU62 Mouse
  • SwissProt: Q920F6 Mouse
  • Unigene: 334176 Human
  • Unigene: 182737 Mouse

  see all

• Alternative names
  CDLS2 antibodyDKFZp686L19178 antibodyDXhXs423e antibody

  DXS423E antibodyKIAA0178 antibodyMGC138332 antibodyOTTHUMP00000061876 antibodyRP6 29D12.1 antibodySB1.8 antibodySegregation of mitotic chromosomes 1 antibodySegregation of mitotic chromosomes like 1 antibodySMC 1 antibodySMC protein 1B antibodySMC-1-beta antibodySMC-1B antibodySMC1 antibodySMC1A antibodySMC1alpha antibodySMC1alpha protein antibodySMC1B antibodySMC1B_HUMAN antibodySMC1BETA antibodySMC1beta protein antibodySMC1L1 antibodySMC1L2 antibodySMCB antibodyStructural maintenance of chromosome 1 like 1 protein antibodyStructural maintenance of chromosome 1 like 2 protein antibodyStructural maintenance of chromosomes 1A antibodyStructural maintenance of chromosomes 1B antibodyStructural maintenance of chromosomes protein 1B antibody

  see all

Anti-SMC1 (phospho S966) antibody images

• 

  Western blot - SMC1 (phospho S966) antibody (ab73994)
  All lanes : Anti-SMC1 (phospho S966) antibody (ab73994) at 1/500 dilution
  
  Lane 1 : extracts from HUVEC cells treated with etoposide (24uM, 24hours),
  Lane 2 : extracts from HUVEC cells, treated with etoposide (24uM, 24hours), with immunising phosphopeptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 143 kDa
  Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  Additional bands at : <72 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S966) antibody (ab73994)
  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using 1/50 ab73994. Left: untreated sample. Right: sample treated with immunising phosphopeptide.