Overview

  • Product nameAnti-SMC1L2 antibodySee all SMC1L2 primary antibodies ...
  • Description
    Mouse monoclonal to SMC1L2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 551-661 of Human SMC1L2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab57086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMeiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.
    • Sequence similaritiesBelongs to the SMC family. SMC1 subfamily.
    • DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 or REC8 protein, forming a ring structure.
    • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. In prophase I stage of meiosis, localizes along the AE of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. Remains chromatin associated at the centromeres up to metaphase II. At anaphase II, dissociates from centromeres, allowing chromosomes segregation.
    • Information by UniProt
    • Database links
    • Alternative names
      • bK268H5 antibody
      • SMC protein 1B antibody
      • SMC-1-beta antibody
      • SMC-1B antibody
      • SMC1 beta antibody
      • SMC1B antibody
      • SMC1B_HUMAN antibody
      • SMC1beta antibody
      • SMC1beta protein antibody
      • Smc1l2 antibody
      • Structural maintenance of chromosomes 1-like 2 antibody
      • Structural maintenance of chromosomes 1B antibody
      • Structural maintenance of chromosomes protein 1B antibody
      see all

    Anti-SMC1L2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57086 SMC1L2 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

    References for Anti-SMC1L2 antibody (ab57086)

    ab57086 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab57086.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"