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Overview

  • Product nameSMC3 protein (Tagged-His Tag)See all SMC3 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 233-736 (504 aa) of Human SMC3 with a N terminal His tag; predicted MWt 60 kDa: Uniprot ID = Q9UQE7.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 118 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIER QVRELKTKISAMK EEKEQLSAERQEQIKQRTKLELKAK DLQDELAGNSEQRKRLLKERQKLLEKIE EKQKELAETE PKFNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSK EER DKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEAN KEKNLEQYNKLDQDLNEV KARVEELDRKYYEVKNKKDE LQSERNYLWREENAEQQALAAKREDLEKKQQLL RAATG KAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFEC EPAFYTCV EVTAGNRLFYHIVDSDEVSTKILMEFNKMN LPGEVTFLPLNKLDVRDTAYPET NDAIPMISKLRYNPR FDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGD QVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKL NENLRRNIERINN EIDQLMNQMQQIETQQRKFKASRDS IL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91760 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        BAMBamacanBasement membrane associated chondroitin proteoglycan
        Basement membrane-associated chondroitin proteoglycanBMHCDLS3Chondroitin sulfate proteoglycan 6chondroitin sulfate proteoglycan 6 (bamacan)Chromosome associated polypeptideChromosome-associated polypeptideCSPG 6CSPG6hCAPSMC 3SMC protein 3SMC-3smc3SMC3_HUMANSMC3L1Structural maintenance of chromosome 3Structural maintenance of chromosomes 3Structural maintenance of chromosomes protein 3
      see all
  • FunctionCentral component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • Involvement in diseaseDefects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • Sequence similaritiesBelongs to the SMC family. SMC3 subfamily.
  • DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

    • Target information above from: UniProt accession Q9UQE7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SMC3 protein (Tagged-His Tag) images

    • SDS-PAGE - SMC3 protein (Tagged-His Tag) (ab91760)
      SDS-PAGE - SMC3 protein (Tagged-His Tag) (ab91760)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SMC3 protein (Tagged-His Tag) (ab91760)

    ab91760 has not yet been referenced specifically in any publications.

    Publishing research using ab91760 ? Please let us know so that we can cite the reference in this datasheet

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