SMC3 protein (Tagged-His Tag) (ab91760)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- BAMBamacanBasement membrane associated chondroitin proteoglycan
- Basement membrane-associated chondroitin proteoglycanBMHCDLS3Chondroitin sulfate proteoglycan 6chondroitin sulfate proteoglycan 6 (bamacan)Chromosome associated polypeptideChromosome-associated polypeptideCSPG 6CSPG6hCAPSMC 3SMC protein 3SMC-3smc3SMC3_HUMANSMC3L1Structural maintenance of chromosome 3Structural maintenance of chromosomes 3Structural maintenance of chromosomes protein 3
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
SMC3 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for SMC3 protein (Tagged-His Tag) (ab91760)
ab91760 has not yet been referenced specifically in any publications.