Recombinant Anti-SMCHD1 antibody [EPR12340] (ab175235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12340] to SMCHD1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SMCHD1 antibody [EPR12340]
See all SMCHD1 primary antibodies -
Description
Rabbit monoclonal [EPR12340] to SMCHD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SMCHD1 aa 1800-2000. The exact sequence is proprietary.
Database link: A6NHR9 -
Positive control
- WB: 293T, HepG2, HeLa, and U-87 MG cell lysates. IHC-P: Human lung carcinoma and Human testis tissue. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12340 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 226 kDa.
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IP |
1/30.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 226 kDa. |
IP
1/30. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 23347 Human
- Omim: 614982 Human
- SwissProt: A6NHR9 Human
- Unigene: 8118 Human
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Alternative names
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
see all
Images
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All lanes : Anti-SMCHD1 antibody [EPR12340] (ab175235) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 226 kDa -
Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling SMCHD1 with ab175235 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human testis tissue labeling SMCHD1 with ab175235 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Blocking buffer and concentration: 5% NFDM/TBST.
The 60 kDa band in the IP lane may be caused by cleavage (PMID: 30071896).
All lanes : Anti-SMCHD1 antibody [EPR12340] (ab175235) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2 : HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab175235 in HeLa whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 226,60 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175235 has not yet been referenced specifically in any publications.