Anti-SMEK2 antibody (ab70622)

Overview

• Product nameAnti-SMEK2 antibodySee all SMEK2 primary antibodies ...
• Description
  Rabbit polyclonal to SMEK2
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)

• Positive controlWhole cell lysates from HeLa, 293T and NIH3T3 cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Developmental Biology
  • Post embryonic development
  • Aging

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Forkhead Box
  • FOXO

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• RelevanceSMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
• Cellular localizationCytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
• Database links
  • Entrez Gene: 57223 Human
  • Entrez Gene: 104570 Mouse
  • Omim: 610352 Human
  • SwissProt: Q5MIZ7 Human
  • SwissProt: Q922R5 Mouse
  • Unigene: 516182 Human
  • Unigene: 276386 Mouse

• Alternative names
  FLFL2 antibodyFLJ31474 antibodyKIAA1387 antibody

  PP4R3 beta antibodyPP4R3B antibodyPPP4R3 Beta antibodyPPP4R3B antibodyProtein Phosphatase 4, Regulatory Subunit 3 Beta, antibodyPSY2 antibodySerine/threonine-protein phosphatase 4 regulatory subunit 3B antibodySMEK 2 antibodySMEK homolog 2 antibodySMEK homolog 2 suppressor of mek1 (Dictyostelium) antibodySMEK homolog 2 suppressor of mek1 antibodysmk1 antibody

  see all

Anti-SMEK2 antibody images

• 

  Western blot - SMEK2 antibody (ab70622)
  All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  Lane 5 : NIH3T3 whole cell lysate at 50 µg
  
  
  Predicted band size : 97 kDa
  Observed band size : 97 kDa
  
• 

  Immunoprecipitation - SMEK2 antibody (ab70622)
  Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.