Overview

  • Product nameAnti-SMEK2 antibody
    See all SMEK2 primary antibodies
  • Description
    Rabbit polyclonal to SMEK2
  • Tested applicationsIHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)

  • Positive control
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceSMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
  • Cellular localizationCytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
  • Database links
  • Alternative names
    • FLFL2 antibody
    • FLJ31474 antibody
    • KIAA1387 antibody
    • PP4R3 beta antibody
    • PP4R3B antibody
    • PPP4R3 Beta antibody
    • PPP4R3B antibody
    • Protein Phosphatase 4, Regulatory Subunit 3 Beta, antibody
    • PSY2 antibody
    • Serine/threonine-protein phosphatase 4 regulatory subunit 3B antibody
    • SMEK 2 antibody
    • SMEK homolog 2 antibody
    • SMEK homolog 2 suppressor of mek1 (Dictyostelium) antibody
    • SMEK homolog 2 suppressor of mek1 antibody
    • smk1 antibody
    see all

Anti-SMEK2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMEK2 with ab70622 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.

References for Anti-SMEK2 antibody (ab70622)

ab70622 has not yet been referenced specifically in any publications.

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