Anti-SMEK2 antibody (ab70622)
- Product nameAnti-SMEK2 antibodySee all SMEK2 primary antibodies ...
- DescriptionRabbit polyclonal to SMEK2
- Tested applicationsWB, IP more details
- Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)
- Positive controlWhole cell lysates from HeLa, 293T and NIH3T3 cells.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab70622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
|IP||IP: Use at 2-5 µg/mg of lysate.|
- RelevanceSMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
- Cellular localizationCytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
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Anti-SMEK2 antibody images
All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 97 kDa
Observed band size : 97 kDa
Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.
References for Anti-SMEK2 antibody (ab70622)
ab70622 has not yet been referenced specifically in any publications.