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Product name
Anti-SMUG1 antibody
See all SMUG1 products (4) ...
Description
Goat polyclonal to SMUG1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: PQAFLLGSIHEPA, corresponding to N terminal amino acids 2-14 of SMUG1.
PQAFLLGSIHEPA-C
Epitope
From N terminus
Positive control
Molt-4 lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Western blot - SMUG1 antibody (ab15716)
(enlarge)
Applications
Our Abpromise guarantee covers the use of ab15716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
Target
Function
Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ53HV7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FDG antibody
- HMUDG antibody
- MGC104370 antibody
Database links
- Entrez Gene: 23583 Human
- Omim: 607753 Human
- SwissProt: Q0D2M0 Human
- SwissProt: Q53HV7 Human
- Unigene: 632721 Human
Anti-SMUG1 antibody images:
Western blot - SMUG1 antibody (ab15716)
Predicted band size : 31 kDa
ab15716 at 0.5µg/ml staining SMUG1 in Molt4 lysate by Western blot (ECL)
References for Anti-SMUG1 antibody (ab15716)
This product has been referenced in:
- Zaika E et al. p73 protein regulates DNA damage repair. FASEB J : (2011). WB; Human.Read more (PubMed: 21891782) »
See 1 publication for this product
Publishing research using ab15716? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"