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Product name
Anti-SMURF 2 antibody
See all SMURF 2 products (4) ...
Description
Rabbit polyclonal to SMURF 2
Specificity
This antibody is specific for SMURF 2 (N term).
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human SMURF 2.
Positive control
Mouse brain tissue lysate and breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Target
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Tissue specificity
Widely expressed.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 3 WW domains.
Domain
The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
Post-translational
modifications
Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
Cellular localization
Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
Target information above from: UniProt accessionQ9HAU4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 ubiquitin-protein ligase SMURF2 antibody
- EC 6.3.2. antibody
- hSMURF2 antibody
see all
Database links
- Entrez Gene: 64750 Human
- Entrez Gene: 66313 Mouse
- Omim: 605532 Human
- SwissProt: Q52LL1 Human
- SwissProt: Q9HAU4 Human
- SwissProt: Q5IRE6 Mouse
- SwissProt: Q3TT87 Mouse
- SwissProt: A2A5Z6 Mouse
see all
Anti-SMURF 2 antibody images:
Western blot - SMURF 2 antibody (ab38543)
Anti-SMURF 2 antibody (ab38543) at 1/100 dilution + Mouse brain tissue lysate
Predicted band size : 86 kDa
Observed band size : 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMURF 2 antibody (ab38543)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38543 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-SMURF 2 antibody (ab38543)
This product has been referenced in:
- Chen YL et al. Smad6 inhibits the transcriptional activity of Tbx6 by mediating its degradation. J Biol Chem 284:23481-90 (2009). WB; Mouse.Read more (PubMed: 19561075) »
- Anindya R et al. Damage-induced ubiquitylation of human RNA polymerase II by the ubiquitin ligase Nedd4, but not Cockayne syndrome proteins or BRCA1. Mol Cell 28:386-97 (2007). WB; Human.Read more (PubMed: 17996703) »
See all 2 publications for this product
Publishing research using ab38543? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"